Hosuk:LabNotes/2014-1-27: Difference between revisions
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*Raw: [[Media:MIP_NCIH1975_Mutant_Pos1_Step0_Cy5.tif|Pos1]], [[Media:MIP_NCIH1975_Mutant_Pos2_Step0_Cy5.tif|Pos2]], [[Media:MIP_NCIH1975_Mutant_Pos3_Step0_Cy5.tif|Pos3]] | *Raw: [[Media:MIP_NCIH1975_Mutant_Pos1_Step0_Cy5.tif|Pos1]], [[Media:MIP_NCIH1975_Mutant_Pos2_Step0_Cy5.tif|Pos2]], [[Media:MIP_NCIH1975_Mutant_Pos3_Step0_Cy5.tif|Pos3]] | ||
[[File:NCIH1975_Mutant_Pos1-2-3_1stRolony.png| | [[File:NCIH1975_Mutant_Pos1-2-3_1stRolony.png|1000px]] | ||
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*'''Pos1''' --> Raw : [[Media:MIP_NCIH1975_Mutant_Pos1_Step1_dcProbe1-Cy3_edit_Mark.tif|dcProbe1]], [[Media:MIP_NCIH1975_Mutant_Pos1_Step2_dcProbe2-Cy3_edit_Mark.tif|dcProbe2]], [[Media:MIP_NCIH1975_Mutant_Pos1_Step3_dcProbe3-Cy3_edit_Mark.tif|dcProbe3]], [[Media:MIP_NCIH1975_Mutant_Pos1_Step4_dcProbe4-Cy3_edit_Mark.tif|dcProbe4]] | *'''Pos1''' --> Raw : [[Media:MIP_NCIH1975_Mutant_Pos1_Step1_dcProbe1-Cy3_edit_Mark.tif|dcProbe1]], [[Media:MIP_NCIH1975_Mutant_Pos1_Step2_dcProbe2-Cy3_edit_Mark.tif|dcProbe2]], [[Media:MIP_NCIH1975_Mutant_Pos1_Step3_dcProbe3-Cy3_edit_Mark.tif|dcProbe3]], [[Media:MIP_NCIH1975_Mutant_Pos1_Step4_dcProbe4-Cy3_edit_Mark.tif|dcProbe4]] | ||
[[File:NCIH1975_Mutant_Pos1_dcProbe.png| | [[File:NCIH1975_Mutant_Pos1_dcProbe.png|800px]] | ||
*'''Pos2''' --> Raw : [[Media:MIP_NCIH1975_Mutant_Pos2_Step1_dcProbe1-Cy3_edit_Mark.tif|dcProbe1]], [[Media:MIP_NCIH1975_Mutant_Pos2_Step2_dcProbe2-Cy3_edit_Mark.tif|dcProbe2]], [[Media:MIP_NCIH1975_Mutant_Pos2_Step3_dcProbe3-Cy3_edit_Mark.tif|dcProbe3]], [[Media:MIP_NCIH1975_Mutant_Pos2_Step4_dcProbe4-Cy3_edit_Mark.tif|dcProbe4]] | *'''Pos2''' --> Raw : [[Media:MIP_NCIH1975_Mutant_Pos2_Step1_dcProbe1-Cy3_edit_Mark.tif|dcProbe1]], [[Media:MIP_NCIH1975_Mutant_Pos2_Step2_dcProbe2-Cy3_edit_Mark.tif|dcProbe2]], [[Media:MIP_NCIH1975_Mutant_Pos2_Step3_dcProbe3-Cy3_edit_Mark.tif|dcProbe3]], [[Media:MIP_NCIH1975_Mutant_Pos2_Step4_dcProbe4-Cy3_edit_Mark.tif|dcProbe4]] | ||
[[File:NCIH1975_Mutant_Pos2_dcProbe.png| | [[File:NCIH1975_Mutant_Pos2_dcProbe.png|800px]] | ||
*'''Pos3''' --> Raw : [[Media:MIP_NCIH1975_Mutant_Pos3_Step1_dcProbe1-Cy3_edit_Mark.tif|dcProbe1]], [[Media:MIP_NCIH1975_Mutant_Pos3_Step2_dcProbe2-Cy3_edit_Mark.tif|dcProbe2]], [[Media:MIP_NCIH1975_Mutant_Pos3_Step3_dcProbe3-Cy3_edit_Mark.tif|dcProbe3]], [[Media:MIP_NCIH1975_Mutant_Pos3_Step4_dcProbe4-Cy3_edit_Mark.tif|dcProbe4]] | *'''Pos3''' --> Raw : [[Media:MIP_NCIH1975_Mutant_Pos3_Step1_dcProbe1-Cy3_edit_Mark.tif|dcProbe1]], [[Media:MIP_NCIH1975_Mutant_Pos3_Step2_dcProbe2-Cy3_edit_Mark.tif|dcProbe2]], [[Media:MIP_NCIH1975_Mutant_Pos3_Step3_dcProbe3-Cy3_edit_Mark.tif|dcProbe3]], [[Media:MIP_NCIH1975_Mutant_Pos3_Step4_dcProbe4-Cy3_edit_Mark.tif|dcProbe4]] | ||
[[File:NCIH1975_Mutant_Pos3_dcProbe.png| | [[File:NCIH1975_Mutant_Pos3_dcProbe.png|800px]] |
Latest revision as of 02:06, 28 January 2014
NCIH1975 : Mutation Detection[edit]
- Continued from 1/19
2nd Rolony[edit]
- Annealing Padlock probes, Ampligase, and 2nd RCA fab. --> done at 01/19~01/21
Detection of Mutation[edit]
- Procedure
- Add Cy5-Adapt to detect 1st Rolonies, 3 positions
- Strip (80% Formamide for 15min), Add dcProbe1 (incubate 15min), image
- Strip (80% Formamide for 15min), Add dcProbe2 (incubate 15min), image
- Strip (80% Formamide for 15min), Add dcProbe3 (incubate 15min), image
- Strip (80% Formamide for 15min), Add dcProbe4 (incubate 15min), image
Result[edit]
- Image at 3 different positions
1st Rolony[edit]
- Pos1 : 5151
- Pos2 : 5964
- Pos3 : 6485
File:NCIH1975 Mutant Pos1-2-3 1stRolony.png
Each dcPobes[edit]
- Not many, just a few, 10~20 from 2nd rolonies.
- Need positive control or some information about expected abundance of each gene.
File:NCIH1975 Mutant dcProbeCount.png
- Images of each gene at each position
- Circles are the 2nd Rolony that I've counted.
File:NCIH1975 Mutant Pos1 dcProbe.png
File:NCIH1975 Mutant Pos2 dcProbe.png