Daniel:Notebook/HiResChrPaint/2014-2-22: Difference between revisions
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>Djacobse (Created page with "=Multi-Color FISH (Started 02/08/2014)= Back to Calendar ==Hybridization== '''Sample Matrix''' ...") |
>Djacobse |
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#Denature for 2.5 minutes at 92C; keep humid | #Denature for 2.5 minutes at 92C; keep humid | ||
#Transfer slides to a humidified chamber and hybridize overnight at 37C or 42C | #Transfer slides to a humidified chamber and hybridize overnight at 37C or 42C | ||
Had to put this on hold. See [[Daniel:Notebook/HiResChrPaint/2014-2-23|tomorrow]] |
Revision as of 00:24, 23 February 2014
Multi-Color FISH (Started 02/08/2014)
Hybridization
Sample Matrix
Day 1
- Allow stored samples to warm to room temperature
- Incubate in 2X SSCT +50% (v/v) formamide for 2.5 minutes at 92 C in pre-warmed media
- Incubate in 2X SSCT + 50% formamide at 60C for 20 minutes
- Remove samples and allow to cool to room temperature
- Add 25 uL hybridization cocktail to center
- Add 22x22 #1.5 coverslip; seal with rubber cement
- Allow the rubber cement to air-dry for 5 minutes at room temperature
- Denature for 2.5 minutes at 92C; keep humid
- Transfer slides to a humidified chamber and hybridize overnight at 37C or 42C
Had to put this on hold. See tomorrow