Daniel:Notebook/HiResChrPaint/2014-2-24: Difference between revisions

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>Djacobse
(Created page with "=Multi-Color FISH (Started 02/08/2014)= Back to Calendar ==Hybridization ([[Daniel:Notebook/HiRe...")
 
>Djacobse
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''Day 2''
''Day 2''
#The next day, remove coverslip carefully and wash in pre-warmed 2X SSCT at 60C for 15 minutes
#Incubate in 2X SSCT at room temperature for 10 minutes
#Incubate in 0.2X SSC at room temperature for 10 minutes
#Dry with kimwipes; do not let the surface containing the cells touch the kimwipes
#Add 15 uL DRAQ5 (diluted 1:1000) to center of dish
#Cover with 22x22 #1.5 coverslip; seal with nail polish
##Allow at least 30 minutes for nail polish to dry
##Slides are quite stable for several months at 4C

Revision as of 16:38, 24 February 2014

Multi-Color FISH (Started 02/08/2014)

Back to Calendar

Hybridization (Started 02/23/2014)

Day 2

  1. The next day, remove coverslip carefully and wash in pre-warmed 2X SSCT at 60C for 15 minutes
  2. Incubate in 2X SSCT at room temperature for 10 minutes
  3. Incubate in 0.2X SSC at room temperature for 10 minutes
  4. Dry with kimwipes; do not let the surface containing the cells touch the kimwipes
  5. Add 15 uL DRAQ5 (diluted 1:1000) to center of dish
  6. Cover with 22x22 #1.5 coverslip; seal with nail polish
    1. Allow at least 30 minutes for nail polish to dry
    2. Slides are quite stable for several months at 4C