Song Chen:Daily/NOTES/2014-2-28: Difference between revisions
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>Chensong (Created page with "===Hepatic maturation from high-density DE differentiation=== Setting: Expose 1000 ms, PFBH 406, 10x obj, <font color=red>ALB</font><b>/</b><font color=green>HNF4A</font> {|{{...") |
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===Hepatic maturation from high-density DE differentiation=== | ===Hepatic maturation from high-density DE differentiation=== | ||
Setting: Expose 1000 ms, PFBH 406, 10x obj, | Setting: Expose 1000 ms, PFBH 406, 10x obj, | ||
<font color=red>ALB</font><b>/</b><font color=green>HNF4A</font> | <font color=red>ALB</font><b>/</b><font color=green>HNF4A</font> | ||
{|{{table}} | {|{{table}} | ||
|[[File:022814_iPH_ALB. | |[[File:022814_iPH_ALB.jpg|300px]] | ||
|[[File:022814_iPH_HNF4A. | |[[File:022814_iPH_HNF4A.jpg|300px]] | ||
|[[File:022814_iPH_DAPI. | |[[File:022814_iPH_DAPI.jpg|300px]] | ||
|} | |} | ||
<font color=red>CYP3A4</font><b>/</b><font color=green>PROX1</font> | <font color=red>CYP3A4</font><b>/</b><font color=green>PROX1</font> | ||
{|{{table}} | {|{{table}} | ||
|[[File:022814_iPH_3A4. | |[[File:022814_iPH_3A4.jpg|300px]] | ||
|[[File:022814_iPH_PROX1. | |[[File:022814_iPH_PROX1.jpg|300px]] | ||
|[[File:022814_iPH_DAPI. | |[[File:022814_iPH_DAPI.jpg|300px]] | ||
|} | |} | ||
Revision as of 21:58, 28 February 2014
Hepatic maturation from high-density DE differentiation
Setting: Expose 1000 ms, PFBH 406, 10x obj,
ALB/HNF4A
File:022814 iPH ALB.jpg | File:022814 iPH HNF4A.jpg | File:022814 iPH DAPI.jpg |
CYP3A4/PROX1
File:022814 iPH 3A4.jpg | File:022814 iPH PROX1.jpg | File:022814 iPH DAPI.jpg |
iPH(iPS-derived hepatocyte) maturatioin: DF12/B27/HGF, DMEM/ITS
iPH cells express ALB, CYP3A4, while HNF4A is a little bit low.