Song Chen:Daily/NOTES/2014-2-26: Difference between revisions

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H9 hESCs cultured in naive condition express OCT4,SOX2 and NANOG. While SOX2 and NANOG expression are very clear, OCT4 expression is still dim, and sometimes there are some OCT4 high cells with flatten morphology.
H9 hESCs cultured in naive condition express OCT4,SOX2 and NANOG. While SOX2 and NANOG expression are very clear, OCT4 expression is still dim, and sometimes there are some OCT4 high cells with flatten morphology.
SSEA4 is still unclear, YAP shows majority nuclear localization in hES colonies.

Latest revision as of 22:40, 28 February 2014

Naive condition cultured H9 ES cells[edit]

Setting: Expose 1000 ms, PFBH 406, 10x obj,

NANOG/SOX2

File:022614 NHSM NANOG.png File:022614 NHSM SOX2.png File:022614 NHSM DAPI.png

OCT4/SOX2

File:022614 NHSM OCT4.png File:022614 NHSM SOX2.2.png File:022614 NHSM DAPI.2.png

H9 hESCs cultured in naive condition express OCT4,SOX2 and NANOG. While SOX2 and NANOG expression are very clear, OCT4 expression is still dim, and sometimes there are some OCT4 high cells with flatten morphology.

SSEA4 is still unclear, YAP shows majority nuclear localization in hES colonies.