Kun:LabNotes/ASE/2008-11-26: Difference between revisions

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==Perform capturing experiment with the E27kV2 probe set==
 
                                      A                    B
    PGP1 gDNA (158ng/ul,11/26/08)    4ul                  4ul
    10X buffer                        2ul                  2ul
    E27KV2 Nicking (38nM)            14ul    DpnII (140nM) 8ul
    H2O                                                    6ul
    95C 5min -> cool to 70C at 0.1C/sec -> 70C 6h -> cool to 60C at 0.1C/sec
      -> 60C 48h -> add 2ul SLN mix () -> 60C 2h -> 94C 1min -> 37C 1min ]
      -> add 2u Exo I&III -> 37C 2h -> 90C 5min -> 4C hold

Latest revision as of 02:04, 30 November 2008