Hosuk:LabNotes/2014-3-3: Difference between revisions
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====Result==== | ====Result : Sample fixed with 4% Formalin==== | ||
*More mRNAs than before, as well as more 1st Rolonies | *More mRNAs than before, as well as more 1st Rolonies | ||
*RAB7A were most, and ACTB, and MALAT1 were fewer than other two genes | *RAB7A were most, and ACTB, and MALAT1 were fewer than other two genes | ||
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*Raw: [[Media:MIP_PGP1F-noEGF_Formalin-4_Step1_1stRolony_Laser1-PMT470.tif|1st Rolony]], [[Media:MIP_PGP1F-noEGF_Formalin-4_Step9_ACTB_Laser5-PMT680.tif|ACTB]], [[Media:MIP_PGP1F-noEGF_Formalin-4_Step11_RAB7A_Laser5-PMT680.tif|RAB7A]], [[Media:MIP_PGP1F-noEGF_Formalin-4_Step7_MALAT1_Laser5-PMT700.tif|MALAT1]] | *Raw: [[Media:MIP_PGP1F-noEGF_Formalin-4_Step1_1stRolony_Laser1-PMT470.tif|1st Rolony]], [[Media:MIP_PGP1F-noEGF_Formalin-4_Step9_ACTB_Laser5-PMT680.tif|ACTB]], [[Media:MIP_PGP1F-noEGF_Formalin-4_Step11_RAB7A_Laser5-PMT680.tif|RAB7A]], [[Media:MIP_PGP1F-noEGF_Formalin-4_Step7_MALAT1_Laser5-PMT700.tif|MALAT1]] | ||
[[File:Rolonies_ConfocalImage_2014-03-03.png|800px]] | [[File:Rolonies_ConfocalImage_2014-03-03.png|800px]] | ||
====Result : Sample fixed with 10% Formalin==== | |||
*More mRNAs than before, but fewer than the sample fixed with 4% Formalin | |||
*More even distribution, not localized at nucleus | |||
=====Counts===== | |||
[[File:Counts_Formalin10_2014-03-04.png|400px]] | |||
*Counting was done after aligning | |||
=====Images===== | |||
*Raw: [[Media:MIP_PGP1F-noEGF_Formalin-10_Step1_1stRolony_Laser1-PMT500.tif|1st Rolony]], [[Media:MIP_PGP1F-noEGF_Formalin-10_Step5_ACTB_Laser5-PMT680.tif|ACTB]], [[Media:MIP_PGP1F-noEGF_Formalin-10_Step7_RAB7A_Laser5-PMT700.tif|RAB7A]], [[Media:MIP_PGP1F-noEGF_Formalin-10_Step3_MALAT1_Laser5-PMT700.tif|MALAT1]] | |||
[[File:Rolonies_Fomalin10_ConfocalImage_2014-03-04.png|800px]] |
Revision as of 00:11, 5 March 2014
PGP1F cultured with noEGF media : Result
- Continued from 02/26
- Detection – 1st Rolonies, ACTB, RAB7A and MALAT1 on 1sdt Rolonies
Result : Sample fixed with 4% Formalin
- More mRNAs than before, as well as more 1st Rolonies
- RAB7A were most, and ACTB, and MALAT1 were fewer than other two genes
Counts
- Counting was done after aligning
Images
- Raw: 1st Rolony, ACTB, RAB7A, MALAT1
File:Rolonies ConfocalImage 2014-03-03.png
Result : Sample fixed with 10% Formalin
- More mRNAs than before, but fewer than the sample fixed with 4% Formalin
- More even distribution, not localized at nucleus
Counts
File:Counts Formalin10 2014-03-04.png
- Counting was done after aligning
Images
- Raw: 1st Rolony, ACTB, RAB7A, MALAT1