AlanFung:LabNotes/Rolony/2014-3-5: Difference between revisions

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====Group2 F8(15), F5(14), F6(7), E6(8)====
====Group2 F8(15), F5(14), F6(7), E6(8)====
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8<\hiddentext>
|- style="font-size:12pt;font-weight:bold" align="center"
| width="65" colspan="3" height="15" | Group 2
|- style="font-size:12pt;font-weight:bold" align="center"
| height="15" | Image
| Well
| Mark & Find
|- style="font-size:12pt" align="center"
| height="15" | E
| F8
| align="center" | 15
|- style="font-size:12pt" align="center"
| height="15" | F
| F5
| align="center" | 14
|- style="font-size:12pt" align="center"
| height="15" | G
| F6
| align="center" | 7
|- style="font-size:12pt" align="center"
| height="15" | H
| E6
| align="center" | 8
|}
[[File:ppconcentration_NCHI1975_Group2.jpg|800px]]
[[File:ppconcentration_NCHI1975_Group2.jpg|800px]]


====Group3 C6(9), C7(10), E7(11), F7(12)====
====Group3 C6(9), C7(10), E7(11), F7(12)====
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8<\hiddentext>
|- style="font-size:12pt;font-weight:bold" align="center"
| width="65" colspan="3" height="15" | Group 3
|- style="font-size:12pt;font-weight:bold" align="center"
| height="15" | Image
| Well
| Mark & Find
|- style="font-size:12pt" align="center"
| height="15" | I
| C6
| align="center" | 9
|- style="font-size:12pt" align="center"
| height="15" | J
| C7
| align="center" | 10
|- style="font-size:12pt" align="center"
| height="15" | K
| E7
| align="center" | 11
|- style="font-size:12pt" align="center"
| height="15" | L
| F7
| align="center" | 12
|}
[[File:ppconcentration_NCHI1975_Group3.jpg|800px]]
[[File:ppconcentration_NCHI1975_Group3.jpg|800px]]

Revision as of 22:55, 5 March 2014

Secondary Rolony Generation for Padlock Probe Concentration Test

Samples

  • 96 well plate containing NCIH-1975 cells fixed by Hosuk
  • Location and save location of 12 position in 96 well plate for the experiment
  • High Concentration(100pM)-

Anneal Padlock Probes for 24 hours

  1. 1X Ampligase Buffer
  2. Various padlock probe concentration
  3. 0.5 U/ul Ampligase
  4. Incubate @ 45C for 24 hours
  5. Wrapped in parafilm and put in ziploc with wet towel

Group1 C3(1),D3(2), F3(13), C5(4)

Group 1
Image Well Mark & Find
A C3 1
B D3 2
C F3 13
D C5 4

File:Ppconcentration NCHI1975 Group1.jpg

Group2 F8(15), F5(14), F6(7), E6(8)

Group 2
Image Well Mark & Find
E F8 15
F F5 14
G F6 7
H E6 8

File:Ppconcentration NCHI1975 Group2.jpg

Group3 C6(9), C7(10), E7(11), F7(12)

Group 3
Image Well Mark & Find
I C6 9
J C7 10
K E7 11
L F7 12

File:Ppconcentration NCHI1975 Group3.jpg