Song Chen:Daily/NOTES/2014-3-6: Difference between revisions
Jump to navigation
Jump to search
>Chensong No edit summary |
>Chensong No edit summary |
||
Line 21: | Line 21: | ||
|Bb#6(random integrated) | |Bb#6(random integrated) | ||
|- | |- | ||
|[[File:030614_KI-1_GFP.png| | |[[File:030614_KI-1_GFP.png|200px]] | ||
|[[File:030614_KI-2-4_GFP.png| | |[[File:030614_KI-2-4_GFP.png|200px]] | ||
|[[File:030614_KI-2-6_GFP.png| | |[[File:030614_KI-2-6_GFP.png|200px]] | ||
|[[File:030614_Bb6_GFP.png| | |[[File:030614_Bb6_GFP.png|200px]] | ||
|- | |- | ||
|[[File:030614_KI-1_BF.png| | |[[File:030614_KI-1_BF.png|200px]] | ||
|[[File:030614_KI-2-4_BF.png| | |[[File:030614_KI-2-4_BF.png|200px]] | ||
|[[File:030614_KI-2-6_BF.png| | |[[File:030614_KI-2-6_BF.png|200px]] | ||
|[[File:030614_Bb6_BF.png| | |[[File:030614_Bb6_BF.png|200px]] | ||
|- | |- | ||
|[[File:030614_KI-1_Merge.png| | |[[File:030614_KI-1_Merge.png|200px]] | ||
|[[File:030614_KI-2-4_Merge.png| | |[[File:030614_KI-2-4_Merge.png|200px]] | ||
|[[File:030614_KI-2-6_Merge.png| | |[[File:030614_KI-2-6_Merge.png|200px]] | ||
|[[File:030614_Bb6_Merge.png| | |[[File:030614_Bb6_Merge.png|200px]] | ||
|} | |} |
Revision as of 22:11, 7 March 2014
hES change medium:
- PGP iPS Brainbow knockin #1, #2-4 and #2-6 p2, 2 well*6 well plate in hES/NHSM, respectively;
- H9 p62 2*6 cm+24 well*48-well plate in NHSM
- H9 Bb6 2 well*6 well plate
- PGPs iPS LCB+CRE p62 1*6 cm in hES medium
send H9 in NHSM:
- 1*T25 flask
DE differentition(H9 p62, for methylation analysis-Dinh):
- 3 well*6-well plate, hES at d2, ActA100 d4
check default GFP expression in knockin iPS lines: Setting: Expose 1209 ms, Cerc, 20x obj,GFP/Brightfield, KI(knockin)