Noi/NOTES/2014-6-3: Difference between revisions
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* Performed PAGE size selection in 3 wells of 5-well 12% TBE gel | * Performed PAGE size selection in 3 wells of 5-well 12% TBE gel | ||
[[File:ZhangLab_2 2014-06-06 10hr 08min_5ng-CTT-RRBS_PAGE-SS.jpg| 400px]] | [[File:ZhangLab_2 2014-06-06 10hr 08min_5ng-CTT-RRBS_PAGE-SS.jpg| 400px]] | ||
* Verified 2ul of size-selected libraries in 6% TBE gel | |||
[[File:ZhangLab_2 2014-06-07 22hr 50min_PAGE-verify_NP-RRBS-CTT-5ng.jpg|150px]] | |||
'''Sequencing library ID: NP-RRBS-CTT-5ng, Jun3, 2014''' | '''Sequencing library ID: NP-RRBS-CTT-5ng, Jun3, 2014''' | ||
:- Length: 160-360bp | :- Length: 160-360bp | ||
:- Index: Truseq Ind9-11, 13 | :- Index: Truseq Ind9-11, 13 |
Revision as of 14:23, 8 June 2014
RRBS, tumor tissue sample (colon)
Link to calendar: [[1]]
- Experimental procedur ewill be added
PAGE verification
- Loaded 4ul of 1X bead-purified sequencing libraries (total 60ul) in 12% TBE gel
File:ZhangLab 2 2014-06-06 08hr 41min PQ 5ng-CTT-RRBS 4ul.jpg
PAGE size selection
- Performed PAGE size selection in 3 wells of 5-well 12% TBE gel
File:ZhangLab 2 2014-06-06 10hr 08min 5ng-CTT-RRBS PAGE-SS.jpg
- Verified 2ul of size-selected libraries in 6% TBE gel
File:ZhangLab 2 2014-06-07 22hr 50min PAGE-verify NP-RRBS-CTT-5ng.jpg
Sequencing library ID: NP-RRBS-CTT-5ng, Jun3, 2014
- - Length: 160-360bp
- - Index: Truseq Ind9-11, 13