Daniel:Notebook/HiResChrPaint/2014-6-16: Difference between revisions
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'''Scripts:''' | '''Scripts:''' | ||
[[Media:Oligopaints_Scripts_Manual.pdf|Oligopaints Scripts Manual]] | [[Media:Oligopaints_Scripts_Manual.pdf|Oligopaints Scripts Manual]] | ||
[[Media:]] | [[Media:Probe_mining_hox_example.pdf|Probe Mining Example]] | ||
Setting up the scripts for probe mining. I'm doing this locally on my machine for now. | Setting up the scripts for probe mining. I'm doing this locally on my machine for now, following the instructions in the two pdfs above. | ||
===Setting up a BLAST Database=== | ===Setting up a BLAST Database=== | ||
Required for running the Oligopaints scripts. | Required for running the Oligopaints scripts. |
Revision as of 22:57, 16 June 2014
Probe Design
Using the Oligopaints scripts by Beliveau, my goal is to design a positive/negative control for the FISH probes. The control is to focus on a region of the X chromosome, yet to be determined. Using male cells (such as PGP1), exactly one region should be painted per cell. This serves as a potential positive and negative control.
Probe Mining
Scripts: Oligopaints Scripts Manual Probe Mining Example
Setting up the scripts for probe mining. I'm doing this locally on my machine for now, following the instructions in the two pdfs above.
Setting up a BLAST Database
Required for running the Oligopaints scripts.