Blue:RNA-Seq Experiments:07022014: Difference between revisions
Jump to navigation
Jump to search
>B1lake |
>B1lake |
||
Line 14: | Line 14: | ||
#PBMC 5.02ng/ul | #PBMC 5.02ng/ul | ||
#Neg Cont 1.64ng/ul | #Neg Cont 1.64ng/ul | ||
==NexteraXT== |
Revision as of 15:53, 10 July 2014
PBMC C1 Experiment
Extracted RNA from bulk samples using Zymo microRNA Kit and eluted in 6ul. Determined [RNA] using Qubit:
- CD4+ 26.1 ng/uL
- CD19+ 6.27 ng/uL
- PBMC 95.8 ng/uL
Diluted to 1ng/ul and used 1ng as tube control for C1 run 20140702 (followed standard Smart+randomer C1 tube control protocol with 3 fewer cycles in last stage of PCR)
Qubit quantification of PCR product:
- CD4+ 6.56ng/ul
- CD19+ 7.60ng/ul
- PBMC 5.02ng/ul
- Neg Cont 1.64ng/ul