Hosuk:LabNotes/2014-7-9: Difference between revisions
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>Hosuki78 (Created page with "*LabNote ===NCIH1975, PGP1F with T48 RT Primer=== *Fix NCIH1975 and PGP1F with 4% Formalin at 37C *S1 : T48 RT primer, S2 : rHexamer ====Result==== *Fix...") |
>Hosuki78 No edit summary |
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*Fix NCIH1975 and PGP1F with 4% Formalin at 37C | *Fix NCIH1975 and PGP1F with 4% Formalin at 37C | ||
*S1 : T48 RT primer, S2 : rHexamer | *S1 : T48 RT primer, S2 : rHexamer | ||
====Result==== | ====Result==== | ||
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===Tissue 11th=== | ===Tissue 11th=== | ||
*Tissue 11th: 10um thick, random hexamer, -a-dUTP | *Tissue 11th: 10um thick, random hexamer, -a-dUTP | ||
====Result==== | ====Result==== | ||
Result : Tissue 11th: not as many as expected, similar to 9th result | Result : Tissue 11th: not as many as expected, similar to 9th result |
Revision as of 00:08, 10 July 2014
NCIH1975, PGP1F with T48 RT Primer
- Fix NCIH1975 and PGP1F with 4% Formalin at 37C
- S1 : T48 RT primer, S2 : rHexamer
Result
- Fixing with 4% Formalin at 37C seems better in terms of even distribution of rolonies, which meaning less localized in nuclear
- Rolony with T48 : 30~50% less than rolony with rHex
Tissue 11th
- Tissue 11th: 10um thick, random hexamer, -a-dUTP
Result
Result : Tissue 11th: not as many as expected, similar to 9th result