Hosuk:LabNotes/2014-7-9: Difference between revisions

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>Hosuki78
(Created page with "*LabNote ===NCIH1975, PGP1F with T48 RT Primer=== *Fix NCIH1975 and PGP1F with 4% Formalin at 37C *S1 : T48 RT primer, S2 : rHexamer ====Result==== *Fix...")
 
>Hosuki78
No edit summary
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*Fix NCIH1975 and PGP1F with 4% Formalin at 37C
*Fix NCIH1975 and PGP1F with 4% Formalin at 37C
*S1 : T48 RT primer, S2 : rHexamer
*S1 : T48 RT primer, S2 : rHexamer


====Result====
====Result====
Line 14: Line 13:
===Tissue 11th===
===Tissue 11th===
*Tissue 11th: 10um thick, random hexamer, -a-dUTP
*Tissue 11th: 10um thick, random hexamer, -a-dUTP


====Result====
====Result====
Result : Tissue 11th: not as many as expected, similar to 9th result
Result : Tissue 11th: not as many as expected, similar to 9th result

Revision as of 00:08, 10 July 2014

NCIH1975, PGP1F with T48 RT Primer

  • Fix NCIH1975 and PGP1F with 4% Formalin at 37C
  • S1 : T48 RT primer, S2 : rHexamer

Result

  • Fixing with 4% Formalin at 37C seems better in terms of even distribution of rolonies, which meaning less localized in nuclear
  • Rolony with T48 : 30~50% less than rolony with rHex


Tissue 11th

  • Tissue 11th: 10um thick, random hexamer, -a-dUTP

Result

Result : Tissue 11th: not as many as expected, similar to 9th result