Blue:RNA-Seq Analyses:C1 hNuc 1000 Dataset: Difference between revisions

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*C1 Run Summary:
*C1 Run Summary:
[[File:20chips_Samplelist.png|400px]]
[[File:20chips_Samplelist.png|400px]]
*Primary Mapping/Analysis done by [[Rui:LabNotes/SingleCell/2014-7-3 Rui]] using our pipeline  
*Primary Mapping/Analysis done by [http://genome-tech.ucsd.edu/LabNotes/index.php/Rui:LabNotes/SingleCell/2014-7-3#Primary_analysis_on_20_chips_of_hNuc_BA8 Rui] using our pipeline  
*More in depth analysis by [[Rui:LabNotes/SingleCell/2014-7-4 Rui]]
*More in depth analysis by [http://genome-tech.ucsd.edu/LabNotes/index.php/Rui:LabNotes/SingleCell/2014-7-4 Rui]
 


==Average cDNA Yields Across Runs==
==Average cDNA Yields Across Runs==

Revision as of 18:20, 11 July 2014

Overview

  • C1 Run Summary:

File:20chips Samplelist.png

  • Primary Mapping/Analysis done by Rui using our pipeline
  • More in depth analysis by Rui

Average cDNA Yields Across Runs

File:20 C1 Runs cDNA Yields 2.png


  • Circled experiments represent the same batch of nuclei. There is little effect of freezing the nuclei in 10%DMSO or running the nuclei on a small chip rather than the medium chip. Global levels of cDNA were reduced with adding a nuclei stain/wash step to the loading protocol using the DNA Seq Cell Wash buffer. Also, addition of PolydIdC did globally increase cDNA levels even for the 0_Nuc conditions.