Daniel:Notebook/HiResChrPaint/2014-7-15: Difference between revisions
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>Djacobse (Created page with "=Strand Control (Started 07/08/2014)= Back to Calendar ==Dye Labeling== #Add 100 uL DMSO to via...") |
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#Incubate at 80C for 15 minutes; stop the reaction by plunging the tube into an ice bath | #Incubate at 80C for 15 minutes; stop the reaction by plunging the tube into an ice bath | ||
#Purify using a column (Centri-Sep recommended) | #Purify using a column (Centri-Sep recommended) | ||
[[Category:HRCP]] [[Category:20140708]] [[Category:ULYSIS Label]] |
Revision as of 21:22, 15 July 2014
Strand Control (Started 07/08/2014)
Dye Labeling
- Add 100 uL DMSO to vial containing ULS dye (stable for 6 months at 4C)
- For AlexaFluor 488 label using 5 uL DMSO (stable for 1 month at 4C)
- VORTEX THESE SOLUTIONS VIGOROUSLY BEFORE USE EVERY TIME!
- Resuspend DNA in 20 uL labeling buffer (Component C)
- Denature DNA at 95C for 5 minutes and snap cool on ice; centrifuge briefly
- Add appropriate volume of dye to sample (see table 2); if necessary add labeling buffer to bring volume to 25 uL
- Incubate at 80C for 15 minutes; stop the reaction by plunging the tube into an ice bath
- Purify using a column (Centri-Sep recommended)