Kun:LabNotes/ExonomeSeq: Difference between revisions
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Revision as of 23:00, 4 January 2008
<calendar> name=Kun:LabNotes/ExonomeSeq format=%name/%year-%month-%day date=2007/11/01 view=threemonths </calendar>
<calendar> name=Kun:LabNotes/ExonomeSeq format=%name/%year-%month-%day date=2008/02/01 view=threemonths </calendar>
Probe Design
- Kun:LabNotes/ExonomeSeq/2007-11-15
- One 55k library received on 12/12/2007, OLID: 018274110001, 10pmole, resuspended in 500ul RT-PCR grade water (new vial) -> 20nM x 500ul -> Take 100ul out to a 1.5ml non-stick tube as the working tube.
Sequencing library construction
Random primed
- Forward primer (SolFN6):CGACGCTCTTCCGATCTNNNNNN Tm left = 59.20C
- Reverse primer (SolRN6):CATACGAGCTCTTCCGATCTNNNNNN Tm left = 59.45C