Kun:LabNotes/Haplotyping/2009-1-29: Difference between revisions
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(New page: ==Perform 2nd round MDA on the amplicons from 01/28/2009== *The purpose of this experiment is to generate more DNA for downstream analyses. Only the 16 amplicons from the haploid templates...) |
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RepliPhi Phi-29 (100U/ul) 10ul 30 | RepliPhi Phi-29 (100U/ul) 10ul 30 | ||
Add 200ul MDA mix to each of the tube on cool block, 30C 2h, 85C 3min. | Add 200ul MDA mix to each of the tube on cool block, 30C 2h, 85C 3min. | ||
Make aliquots of amplicons (50ul each) for Infinium Genotyping: | |||
GM18508A | |||
GM18507B | |||
GM18507D | |||
GM18507G | |||
GM20431A | |||
GM20431B | |||
GM20431C | |||
GM20431F | |||
Ethanol precipitation of 150ul amplicons: | |||
GM18508A | |||
GM18507B | |||
GM18507D | |||
GM18507G | |||
GM20431A | |||
GM20431B | |||
GM20431C | |||
GM20431G |
Revision as of 22:23, 29 January 2009
Perform 2nd round MDA on the amplicons from 01/28/2009
- The purpose of this experiment is to generate more DNA for downstream analyses. Only the 16 amplicons from the haploid templates will be re-amplified.
Set up master mix: x 16 10x RepliPhi phi-29 buffer 20ul 320 1uM N6 primer 10ul 160 25mM dNTP 8ul 128 2X SYBR Green I 10ul 160 H2O 142ul 2272 RepliPhi Phi-29 (100U/ul) 10ul 30 Add 200ul MDA mix to each of the tube on cool block, 30C 2h, 85C 3min.
Make aliquots of amplicons (50ul each) for Infinium Genotyping: GM18508A GM18507B GM18507D GM18507G GM20431A GM20431B GM20431C GM20431F Ethanol precipitation of 150ul amplicons: GM18508A GM18507B GM18507D GM18507G GM20431A GM20431B GM20431C GM20431G