Daniel:Notebook/HiResChrPaint/2014-7-18: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Djacobse
No edit summary
>Djacobse
No edit summary
Line 1: Line 1:
=Dystrophin Probes=
=Dystrophin Probes=


[[Daniel:Notebook/HiResChrPaint/2014-7-18|Back to Calendar]]
[[Daniel:Notebook/HiResChrPaint|Back to Calendar]]


The dystrophin probes I designed [[Daniel:Notebook/HiResChrPaint/2014-7-10|last week]] arrived from IDT.  Attached are their specs:
The dystrophin probes I designed [[Daniel:Notebook/HiResChrPaint/2014-7-10|last week]] arrived from IDT.  Attached are their specs:

Revision as of 19:49, 19 July 2014

Dystrophin Probes

Back to Calendar

The dystrophin probes I designed last week arrived from IDT. Attached are their specs:

The oligos arrived as dry oligos in a plate. The calculations I made are in the following text file:

Oligo Pool Synthesis

  1. Resuspend each oligo to make them all 1 mM (See Plate Specs for values)
  2. Combine 2 uL from each oligo
    1. Makes 12.5 ug/uL total pool
    2. Total volume will be 64 uL
  3. Add 742.4 uL nfH2O, dilutes to 1 ug/uL total pool