Kun:LabNotes/ExonomeSeq: Difference between revisions

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2007
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2008
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Revision as of 23:00, 4 January 2008

2007 <calendar> name=Kun:LabNotes/ExonomeSeq format=%name/%year-%month-%day date=2007/11/01 view=threemonths </calendar> 2008 <calendar> name=Kun:LabNotes/ExonomeSeq format=%name/%year-%month-%day date=2008/02/01 view=threemonths </calendar>

Probe Design

  • Kun:LabNotes/ExonomeSeq/2007-11-15
  • One 55k library received on 12/12/2007, OLID: 018274110001, 10pmole, resuspended in 500ul RT-PCR grade water (new vial) -> 20nM x 500ul -> Take 100ul out to a 1.5ml non-stick tube as the working tube.

Sequencing library construction

Random primed

  • Forward primer (SolFN6):CGACGCTCTTCCGATCTNNNNNN Tm left = 59.20C
  • Reverse primer (SolRN6):CATACGAGCTCTTCCGATCTNNNNNN Tm left = 59.45C

USER-Lig