Matt:LabNotes/2014-8-4: Difference between revisions
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>Mzcai (Created page with "==Agi26k0gap Probe Production Continued== *Continued from: Matt:LabNotes/2014-7-22 **Thaw and combine the 7 tubes of 200ul that were stored at -20C ===TBU Gel Check=== *2...") |
>Mzcai mNo edit summary |
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*Undigested sample: 10nM 1st round amplicon | *Undigested sample: 10nM 1st round amplicon | ||
[[]] | [[File:2014-08-04_Agi26k0gapDigestCheck.jpg]] | ||
*Brightest band below 200bp as expected | *Brightest band below 200bp as expected | ||
Revision as of 02:08, 5 August 2014
Agi26k0gap Probe Production Continued
- Continued from: Matt:LabNotes/2014-7-22
- Thaw and combine the 7 tubes of 200ul that were stored at -20C
TBU Gel Check
- 2ul Sample + 2ul 2X Urea Loading Dye
- 0.5ul Low Mass Ladder + 1.5ul H2O + 2ul 2X Urea Loading Dye
- Digested sample
- Undigested sample: 10nM 1st round amplicon
File:2014-08-04 Agi26k0gapDigestCheck.jpg
- Brightest band below 200bp as expected
Column Purification
- Used Zymo ssDNA/RNA columns
- Total Eluted: ul
- Nanodrop quant: ng/ul
- Yield: ng/ul x ul = ug -->