Daniel:Notebook/HiResChrPaint/2014-8-6: Difference between revisions

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>Djacobse
>Djacobse
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==Microscopy==
==Microscopy==
First I'll test whether the [[Daniel:Notebook/HiResChrPaint/2014-8-5|samples]] can be taken directly to the microscope, with the hybridization buffer still on.  This will produce a higher background noise, but will hopefully still result in strong areas of signal distinguishable from the background.  Since this is just a test, I'll only use the positive control (sample D, Cot1-488) for now, and I'll investigate the others pending successful trial.
===Sample D===


<gallery>
<gallery>
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File:20140805_Cot1-488_PreWash.lif_Cot1488_2_ch00.jpg|Cells stained with Cot1, Alexa 488 (green), field 2
File:20140805_Cot1-488_PreWash.lif_Cot1488_2_ch00.jpg|Cells stained with Cot1, Alexa 488 (green), field 2
</gallery>
</gallery>
As expected, the background is definitely higher, but the signal is also stronger.  It will be interesting to do a wash on these samples, and then examine them after.  Given the success, I'm going to return the positive control to the incubator and I'll take snapshots of the other 3 samples.

Revision as of 21:29, 6 August 2014

No Wash FISH

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Microscopy

First I'll test whether the samples can be taken directly to the microscope, with the hybridization buffer still on. This will produce a higher background noise, but will hopefully still result in strong areas of signal distinguishable from the background. Since this is just a test, I'll only use the positive control (sample D, Cot1-488) for now, and I'll investigate the others pending successful trial.

Sample D

As expected, the background is definitely higher, but the signal is also stronger. It will be interesting to do a wash on these samples, and then examine them after. Given the success, I'm going to return the positive control to the incubator and I'll take snapshots of the other 3 samples.