Daniel:Notebook/HiResChrPaint/2014-8-12: Difference between revisions
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>Djacobse (Created page with "=FISH with DAPI (Started 08/10/2014= Back to Calendar ==Hybridization== #Remove coverslip car...") |
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=FISH with DAPI ([[Daniel:Notebook/HiResChrPaint/2014-08-10|Started 08/10/2014]]= | =FISH with DAPI ([[Daniel:Notebook/HiResChrPaint/2014-08-10|Started 08/10/2014]])= | ||
[[Daniel:Notebook/HiResChrPaint|Back to Calendar]] | [[Daniel:Notebook/HiResChrPaint|Back to Calendar]] |
Revision as of 18:48, 12 August 2014
FISH with DAPI (Started 08/10/2014)
Hybridization
- Remove coverslip carefully and wash in pre-warmed 2X SSCT at 60C for 15 minutes
- Incubate in 2X SSCT at room temperature for 10 minutes
- Incubate in 0.2X SSC at room temperature for 10 minutes
- Dry with kimwipes; do not let the surface containing the cells touch the kimwipes
- Add 15 uL DRAQ5 (diluted 1:1000) to center of dish
- Cover with 22x22 #1.5 coverslip; seal with nail polish
- Allow at least 30 minutes for nail polish to dry
- Slides are quite stable for several months at 4C