Hosuk:LabNotes/2014-8-10: Difference between revisions
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>Hosuki78 (Created page with "*LabNote ===Cancer Mutation detection=== ====Sample==== *Sample **NCIH1975_S1_2014-08-05 : -dUTP, rHex 45C **NCIH1975_S2_2014-08-03 : +dUTP, rHex ...") |
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===Cancer Mutation detection=== | ===Cancer Mutation detection=== | ||
==== | ====Decoding, and Analyzing==== | ||
* | *Images | ||
** | **ch00 - dcProbe[#]-488 | ||
** | **ch01 - dcProbe[#]-Cy3 | ||
**ch02 - Bright field | |||
*Steps | |||
**Step1 : dcProbe0-488 | |||
**Step2 : dcProbe0-Cy3 | |||
**Step3 : dcProbe1-488 | |||
**Step4 : dcProbe1-Cy3 | |||
**... | |||
**Step9 : dcProbe4-488 | |||
**Step10 : dcProbe4-Cy3 | |||
*I've taken images from 3 different positions (Pos1, Pos2, and Pos3) | |||
**However the result that successfully analyzed was from only Pos1 | |||
*Procedure | |||
* | *#Figure out the offset pixels with BF image | ||
* | **Pos1 | ||
* | **Standard : Step3 | ||
* | **Step1 : X = 1, Y = -25 | ||
** | **Step2 : X = 0, Y = -8 | ||
**Step3 : X = 0, Y = 0 | |||
**Step4 : X = -8, Y = 2 | |||
**Step5 : X = -8, Y = 9 | |||
*#Make maximum intensity projection(MIP) images at each step | |||
*# | |||
Revision as of 23:11, 12 August 2014
Cancer Mutation detection
Decoding, and Analyzing
- Images
- ch00 - dcProbe[#]-488
- ch01 - dcProbe[#]-Cy3
- ch02 - Bright field
- Steps
- Step1 : dcProbe0-488
- Step2 : dcProbe0-Cy3
- Step3 : dcProbe1-488
- Step4 : dcProbe1-Cy3
- ...
- Step9 : dcProbe4-488
- Step10 : dcProbe4-Cy3
- I've taken images from 3 different positions (Pos1, Pos2, and Pos3)
- However the result that successfully analyzed was from only Pos1
- Procedure
- Figure out the offset pixels with BF image
- Pos1
- Standard : Step3
- Step1 : X = 1, Y = -25
- Step2 : X = 0, Y = -8
- Step3 : X = 0, Y = 0
- Step4 : X = -8, Y = 2
- Step5 : X = -8, Y = 9
- Make maximum intensity projection(MIP) images at each step