Hosuk:LabNotes/2014-8-10: Difference between revisions
Jump to navigation
Jump to search
>Hosuki78 No edit summary |
>Hosuki78 No edit summary |
||
Line 25: | Line 25: | ||
*Procedure | *Procedure | ||
*#Figure out the offset pixels with BF image | *#Figure out the offset pixels with BF image | ||
**Pos1 | *#*Pos1 | ||
**Standard : Step3 | *#*Standard : Step3 | ||
**Step1 : X = 1, Y = -25 | *#*Step1 : X = 1, Y = -25 | ||
**Step2 : X = 0, Y = -8 | *#*Step2 : X = 0, Y = -8 | ||
**Step3 : X = 0, Y = 0 | *#*Step3 : X = 0, Y = 0 | ||
**Step4 : X = -8, Y = 2 | *#*Step4 : X = -8, Y = 2 | ||
**Step5 : X = -8, Y = 9 | *#*Step5 : X = -8, Y = 9 | ||
*#Make maximum intensity projection(MIP) images at each step | *#Make maximum intensity projection(MIP) images at each step |
Revision as of 23:11, 12 August 2014
Cancer Mutation detection
Decoding, and Analyzing
- Images
- ch00 - dcProbe[#]-488
- ch01 - dcProbe[#]-Cy3
- ch02 - Bright field
- Steps
- Step1 : dcProbe0-488
- Step2 : dcProbe0-Cy3
- Step3 : dcProbe1-488
- Step4 : dcProbe1-Cy3
- ...
- Step9 : dcProbe4-488
- Step10 : dcProbe4-Cy3
- I've taken images from 3 different positions (Pos1, Pos2, and Pos3)
- However the result that successfully analyzed was from only Pos1
- Procedure
- Figure out the offset pixels with BF image
- Pos1
- Standard : Step3
- Step1 : X = 1, Y = -25
- Step2 : X = 0, Y = -8
- Step3 : X = 0, Y = 0
- Step4 : X = -8, Y = 2
- Step5 : X = -8, Y = 9
- Figure out the offset pixels with BF image
- Make maximum intensity projection(MIP) images at each step