Sam:LabNotes/Microbione/2009-2-5: Difference between revisions
Jump to navigation
Jump to search
>Sam Chiang |
>Sam Chiang |
||
Line 11: | Line 11: | ||
==Results== | ==Results== | ||
[[Image:sam020409.jpg|frame| | [[Image:sam020409.jpg|frame|50px|'''FIG.1 Realtime amplification plot (no log in flourescent)''']] | ||
[[Image:sam020409-log.jpg|frame| | [[Image:sam020409-log.jpg|frame|50px|'''FIG.2 Realtime amplification plot (with log in flourescent)''']] | ||
==Discussion== | ==Discussion== |
Revision as of 20:54, 5 February 2009
Follow up: Testing human 18S and Bac (E. coli) 16S primers using realtime PCR
Exp. Design
Template gDNA H2O -------- --- Primer: 18S-211 #1 #2 #3 (#1, #2 - replicates) 18S-306 #4 #5 #6 (#4, #5 - replicates)
Results
Discussion
- The amplification curves are not distinguishable (Sample vs. Blank) within 20 clycles.
- The amplifications cycless were not enough?
- The amplifications are not working?
- Primers' specificity? binding affinity?
- Concentration of templates?