Daniel:Notebook/RNAFISH/2014-8-29: Difference between revisions

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Following [[Daniel:Protocols/TissueFISH|Tissue FISH Protocol]]:
Following [[Daniel:Protocols/TissueFISH|Tissue FISH Protocol]]:


#Fix tissue pieces of an appropriate size in freshly prepared 4% formaldehyde/PBS for 4–24 h
#Fix tissue pieces of an appropriate size in freshly prepared 4% formaldehyde/PBS overnight at 4 C (start time 2pm)
#Wash the tissue in PBS, 3 × 30 min. Tissue can be stored in PBS for few days at +4°C
#Continued [[Daniel:RNAFISH/2014-8-30|tomorrow]]

Revision as of 23:19, 29 August 2014

Trial Run

Back to Calendar

Tissue Section Preparation

Following Tissue FISH Protocol:

  1. Fix tissue pieces of an appropriate size in freshly prepared 4% formaldehyde/PBS overnight at 4 C (start time 2pm)
  2. Continued tomorrow