AlanFung:LabNotes/2014/2014-9-5: Difference between revisions
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{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8<\hiddentext> | {| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8<\hiddentext> | ||
|- style="background-color:#BFBFBF" align="center" | |- style="background-color:#BFBFBF" align="center" | ||
| width="151" height=" | | width="151" height="14" | Component | ||
| width="65" | 1 Library | | width="65" | 1 Library | ||
| width="113" | 15 Libraries + 5% | |||
|- align="center" | |- align="center" |
Revision as of 22:00, 4 September 2014
KAPA Library Preparation Kit illumina Platforms
Parts
- Kapa Library Preparation Kit illumina platforms KR0935 V1.13
Workflow
End Repair Reaction Setup
Component | 1 Library | 15 Libraries + 5% |
End Repair Master Mix: | ||
Water | 8 | 126 |
10X Kapa End Repair Buffer | 7 | 110.25 |
Kapa End Repair Enzyme Mix | 5 | 78.75 |
Total master mix volume | 20 | 315 |
Fragmented, double-stranded DNA | 50ul |
End Repair Master Mix | 20ul |
Total rection Mix | 70ul |
- Mix, and incubate at 20C for 30mins
- Proceed immediately to the next step