AlanFung:LabNotes/2014/2014-9-5: Difference between revisions

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>Alan6017518
>Alan6017518
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{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8<\hiddentext>
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8<\hiddentext>
|- style="background-color:#BFBFBF" align="center"
|- style="background-color:#BFBFBF" align="center"
| width="151" height="28" | Component
| width="151" height="14" | Component
| width="65" | 1 Library
| width="65" | 1 Library
"| width=""113"" | 15 Libraries
| width="113" | 15 Libraries + 5%
(+5% excess)"


|-  align="center"
|-  align="center"

Revision as of 22:00, 4 September 2014

KAPA Library Preparation Kit illumina Platforms

Parts

  • Kapa Library Preparation Kit illumina platforms KR0935 V1.13

Workflow

File:09052014 workflow.jpg

End Repair Reaction Setup

Component 1 Library 15 Libraries + 5%
End Repair Master Mix:    
Water 8 126
10X Kapa End Repair Buffer 7 110.25
Kapa End Repair Enzyme Mix 5 78.75
Total master mix volume 20 315
Fragmented, double-stranded DNA 50ul
End Repair Master Mix 20ul
Total rection Mix 70ul
  • Mix, and incubate at 20C for 30mins
  • Proceed immediately to the next step

End Repair Cleanup