AlanFung:LabNotes/2014/2014-9-8: Difference between revisions
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>Alan6017518 (Created page with "===qPCR Test to verifiy End repair, A-tailing & Adapter Ligation=== {| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconvers...") |
>Alan6017518 |
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| height="14" | Adapter ligated DNA | | height="14" | Adapter ligated DNA | ||
| align="center" | 1 | | align="center" | 1 | ||
| align="center" | 19. | | align="center" | 19.6 | ||
|- align="center" | |- align="center" | ||
| height="14" | 10uM PCR_F | | height="14" | 10uM PCR_F | ||
| align="center" | 0.5 | | align="center" | 0.5 | ||
| align="center" | 9. | | align="center" | 9.8 | ||
|- align="center" | |- align="center" | ||
| height="14" | 10uM PCR_R.Ind2 | | height="14" | 10uM PCR_R.Ind2 | ||
| align="center" | 0.5 | | align="center" | 0.5 | ||
| align="center" | 9. | | align="center" | 9.8 | ||
|- align="center" | |- align="center" | ||
| height="14" | 2x iQ MM | | height="14" | 2x iQ MM | ||
| align="center" | 12.5 | | align="center" | 12.5 | ||
| align="center" | 244. | | align="center" | 244.6 | ||
|- align="center" | |- align="center" | ||
| height="14" | Water | | height="14" | Water | ||
| align="center" | 10.5 | | align="center" | 10.5 | ||
| align="center" | 205. | | align="center" | 205.5 | ||
|- align="center" | |- align="center" | ||
| height="14" | Total master mix volume | | height="14" | Total master mix volume | ||
| align="center" | 25 | | align="center" | 25 | ||
| align="center" | 489. | | align="center" | 489.3 | ||
|} | |} |
Revision as of 17:15, 8 September 2014
qPCR Test to verifiy End repair, A-tailing & Adapter Ligation
Component | 1 Library | 19 Libraries + 3% |
qPCR Master Mix: | ||
Adapter ligated DNA | 1 | 19.6 |
10uM PCR_F | 0.5 | 9.8 |
10uM PCR_R.Ind2 | 0.5 | 9.8 |
2x iQ MM | 12.5 | 244.6 |
Water | 10.5 | 205.5 |
Total master mix volume | 25 | 489.3 |