AlanFung:LabNotes/2014/2014-9-8: Difference between revisions

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>Alan6017518
>Alan6017518
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|-  align="center"
|-  align="center"
| height="14" | 2x iQ MM
| height="14" | 2X Kapa Fast
| align="center" | 12.5
| align="center" | 12.5
| align="center" | 244.6
| align="center" | 244.6
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|}
|}
*98C 2min --> (98C 15sec -> 64C 20sec -> 72C 30sec) x 12 cycles --> 72C 3min -->  15C Hold
*98C 2min --> (98C 15sec -> 64C 20sec -> 72C 30sec) x 12 cycles --> 72C 3min -->  15C Hold
==PAGE Gel Verification==
==PAGE Gel Verification==

Revision as of 20:12, 8 September 2014

qPCR Test to verifiy End repair, A-tailing & Adapter Ligation

Component 1 Library 19 Libraries + 3%
qPCR Master Mix:    
Adapter ligated DNA 1 19.6
10uM PCR_F 0.5 9.8
10uM PCR_R.Ind2 0.5 9.8
2X Kapa Fast 12.5 244.6
Water 10.5 205.5
Total master mix volume 25 489.3
  • 98C 2min --> (98C 15sec -> 64C 20sec -> 72C 30sec) x 12 cycles --> 72C 3min --> 15C Hold

PAGE Gel Verification