Noi/NOTES/2014-8-18: Difference between revisions
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>Noi (Created page with "= Preparation of WGBS libraries of small fragment DNA in cancer patient plasma samples (no DNA fragmentation step, REPEAT with dAdGdCdT for end repair) = [[noi:DMR220k_LabNote...") |
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* [[Noi/NOTES/2014-8-3|'''Continue from''']] | * [[Noi/NOTES/2014-8-3|'''Continue from''']] | ||
* There is one sample change for sample in colon cancer in 2014-08-03 note. '''Sample 6-P-6 was replace with sample 6-P-10.''' | * There is one sample change for sample in colon cancer in 2014-08-03 note. '''Sample 6-P-6 was replace with sample 6-P-10.''' | ||
== 1) End-repair/dA-tailing == | |||
* Incubate plasma DNA with 5U Klenow fragment, exo- and dA:dC:dG:dT (final conc. 1:0.1:0.1:0.1mM) in total volume 20ul |
Revision as of 19:27, 13 September 2014
Preparation of WGBS libraries of small fragment DNA in cancer patient plasma samples (no DNA fragmentation step, REPEAT with dAdGdCdT for end repair)
- Continue from
- There is one sample change for sample in colon cancer in 2014-08-03 note. Sample 6-P-6 was replace with sample 6-P-10.
1) End-repair/dA-tailing
- Incubate plasma DNA with 5U Klenow fragment, exo- and dA:dC:dG:dT (final conc. 1:0.1:0.1:0.1mM) in total volume 20ul