Noi/NOTES/2014-8-18: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Noi
(Created page with "= Preparation of WGBS libraries of small fragment DNA in cancer patient plasma samples (no DNA fragmentation step, REPEAT with dAdGdCdT for end repair) = [[noi:DMR220k_LabNote...")
 
>Noi
mNo edit summary
Line 3: Line 3:
*  [[Noi/NOTES/2014-8-3|'''Continue from''']]
*  [[Noi/NOTES/2014-8-3|'''Continue from''']]
* There is one sample change for sample in colon cancer in 2014-08-03 note. '''Sample 6-P-6 was replace with sample 6-P-10.'''
* There is one sample change for sample in colon cancer in 2014-08-03 note. '''Sample 6-P-6 was replace with sample 6-P-10.'''
== 1) End-repair/dA-tailing ==
* Incubate plasma DNA with 5U Klenow fragment, exo-  and dA:dC:dG:dT (final conc. 1:0.1:0.1:0.1mM) in total volume 20ul

Revision as of 19:27, 13 September 2014

Preparation of WGBS libraries of small fragment DNA in cancer patient plasma samples (no DNA fragmentation step, REPEAT with dAdGdCdT for end repair)

Back to calendar

  • Continue from
  • There is one sample change for sample in colon cancer in 2014-08-03 note. Sample 6-P-6 was replace with sample 6-P-10.

1) End-repair/dA-tailing

  • Incubate plasma DNA with 5U Klenow fragment, exo- and dA:dC:dG:dT (final conc. 1:0.1:0.1:0.1mM) in total volume 20ul