Arichard:Notebook/bulk150125: Difference between revisions
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>Andrew (Created page with "==January 25, 2015 Bulk Nextera== Bulk Nextera from [http://genome-tech.ucsd.edu/LabNotes/index.php/Arichard:Notebook/bulk150122|January 22] worked on 2/3 samples (SW480 2N F...") |
>Andrew |
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{| {{table}} | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"|'''5 libraries''' | | align="center" style="background:#f0f0f0;"|'''5 libraries''' | ||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''6 barcodes''' | | align="center" style="background:#f0f0f0;"|'''6 barcodes''' | ||
|- | |- | ||
| QJ11-bulk-03Feb15-nxt14m||14 or 15 | | QJ11-bulk-03Feb15-nxt14m||||14 or 15 | ||
|- | |- | ||
| QJ12-bulk-03Feb15-nxt15m||15 or 16 | | QJ12-bulk-03Feb15-nxt15m||||15 or 16 | ||
|- | |- | ||
| QJ13-bulk-03Feb15-nxt16m||16 or 17 | | QJ13-bulk-03Feb15-nxt16m||||16 or 17 | ||
|- | |- | ||
| QJ14-bulk-03Feb15-nxt17m||17 or 18 | | QJ14-bulk-03Feb15-nxt17m||||17 or 18 | ||
|- | |- | ||
| QJ15-bulk-03Feb15-nxt18m||18 or 19 | | QJ15-bulk-03Feb15-nxt18m||||18 or 19 | ||
|- | |- | ||
| | | | ||
|} | |} | ||
The m after the index (e.g., nxt14m) denotes "maybe", meaning that it could be either 14 or 15. | The m after the index (e.g., nxt14m) denotes "maybe", meaning that it could be either 14 or 15. |
Revision as of 23:19, 4 February 2015
January 25, 2015 Bulk Nextera
Bulk Nextera from 22 worked on 2/3 samples (SW480 2N Full), so I decided to continue with all the SW480 (including the 2N Full again to get at least N = 3), as well as the lymphocytes (all sorted October 21, 2014).
File:Bulk150125Slide1.jpg File:Bulk150125Slide2.jpg File:Bulk150125Slide3.jpg File:Bulk150125Slide4.jpg
Qiming likely added 2 barcodes to the same sample, so we need to figure out which is which. I will try to spike in the suspect libraries to different lanes to determine which barcodes belong to which libraries.
5 libraries | ' | 6 barcodes |
QJ11-bulk-03Feb15-nxt14m | 14 or 15 | |
QJ12-bulk-03Feb15-nxt15m | 15 or 16 | |
QJ13-bulk-03Feb15-nxt16m | 16 or 17 | |
QJ14-bulk-03Feb15-nxt17m | 17 or 18 | |
QJ15-bulk-03Feb15-nxt18m | 18 or 19 | |
The m after the index (e.g., nxt14m) denotes "maybe", meaning that it could be either 14 or 15.