Arichard:Notebook/bulk150125: Difference between revisions

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>Andrew
(Created page with "==January 25, 2015 Bulk Nextera== Bulk Nextera from [http://genome-tech.ucsd.edu/LabNotes/index.php/Arichard:Notebook/bulk150122|January 22] worked on 2/3 samples (SW480 2N F...")
 
>Andrew
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{| {{table}}
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''5 libraries'''
| align="center" style="background:#f0f0f0;"|'''5 libraries'''
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|'''6 barcodes'''
| align="center" style="background:#f0f0f0;"|'''6 barcodes'''
|-
|-
| QJ11-bulk-03Feb15-nxt14m||14 or 15
| QJ11-bulk-03Feb15-nxt14m||||14 or 15
|-
|-
| QJ12-bulk-03Feb15-nxt15m||15 or 16
| QJ12-bulk-03Feb15-nxt15m||||15 or 16
|-
|-
| QJ13-bulk-03Feb15-nxt16m||16 or 17
| QJ13-bulk-03Feb15-nxt16m||||16 or 17
|-
|-
| QJ14-bulk-03Feb15-nxt17m||17 or 18
| QJ14-bulk-03Feb15-nxt17m||||17 or 18
|-
|-
| QJ15-bulk-03Feb15-nxt18m||18 or 19
| QJ15-bulk-03Feb15-nxt18m||||18 or 19
|-
|-
|  
|  
|}
|}
The m after the index (e.g., nxt14m) denotes "maybe", meaning that it could be either 14 or 15.
The m after the index (e.g., nxt14m) denotes "maybe", meaning that it could be either 14 or 15.

Revision as of 23:19, 4 February 2015

January 25, 2015 Bulk Nextera

Bulk Nextera from 22 worked on 2/3 samples (SW480 2N Full), so I decided to continue with all the SW480 (including the 2N Full again to get at least N = 3), as well as the lymphocytes (all sorted October 21, 2014).

File:Bulk150125Slide1.jpg File:Bulk150125Slide2.jpg File:Bulk150125Slide3.jpg File:Bulk150125Slide4.jpg

Qiming likely added 2 barcodes to the same sample, so we need to figure out which is which. I will try to spike in the suspect libraries to different lanes to determine which barcodes belong to which libraries.

5 libraries ' 6 barcodes
QJ11-bulk-03Feb15-nxt14m 14 or 15
QJ12-bulk-03Feb15-nxt15m 15 or 16
QJ13-bulk-03Feb15-nxt16m 16 or 17
QJ14-bulk-03Feb15-nxt17m 17 or 18
QJ15-bulk-03Feb15-nxt18m 18 or 19

The m after the index (e.g., nxt14m) denotes "maybe", meaning that it could be either 14 or 15.