Blue:RNA-Seq Experiments:03062015: Difference between revisions

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(Created page with "== C1 Tube Controls == *Testing the quality of RNA for the following C1 runs: # 20150205A: Naac bulk sorted nuclei (MSSM) # 20130223A: GTEX Patient 10A Nuclei combined aliqu...")
 
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# 20130223B: GTEX Patient 10B Nuclei combined aliquots
# 20130223B: GTEX Patient 10B Nuclei combined aliquots


* RNA extracted using Zymo micro RNA kit
* RNA extracted using Zymo micro RNA kit (eluted in 6ul)
 
 
RNA Concentrations:
 
# Naac Bulk sort - 18.2ng/ul
# GTEX 10A Bulk Sort (combined 5 aliquots) - 5.17ng/ul
# GTEX 10B Bulk Sort (combined 5 aliquots) - 5.10ng/ul
 
Ran Bioanalyzer using Pico chip and comparing to BA8 sort pool RNA ([[http://genome-tech.ucsd.edu/LabNotes/index.php/Blue:RNA-Seq_Experiments:04302014]]:
 
[[media:RNA_Pico_2015-03-06_14-36-22.pdf]]

Revision as of 01:10, 7 March 2015

C1 Tube Controls

  • Testing the quality of RNA for the following C1 runs:
  1. 20150205A: Naac bulk sorted nuclei (MSSM)
  2. 20130223A: GTEX Patient 10A Nuclei combined aliquots
  3. 20130223B: GTEX Patient 10B Nuclei combined aliquots
  • RNA extracted using Zymo micro RNA kit (eluted in 6ul)


RNA Concentrations:

  1. Naac Bulk sort - 18.2ng/ul
  2. GTEX 10A Bulk Sort (combined 5 aliquots) - 5.17ng/ul
  3. GTEX 10B Bulk Sort (combined 5 aliquots) - 5.10ng/ul

Ran Bioanalyzer using Pico chip and comparing to BA8 sort pool RNA ([[1]]:

media:RNA_Pico_2015-03-06_14-36-22.pdf