Blue:RNA-Seq Experiments:03062015: Difference between revisions
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>B1lake (Created page with "== C1 Tube Controls == *Testing the quality of RNA for the following C1 runs: # 20150205A: Naac bulk sorted nuclei (MSSM) # 20130223A: GTEX Patient 10A Nuclei combined aliqu...") |
>B1lake No edit summary |
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# 20130223B: GTEX Patient 10B Nuclei combined aliquots | # 20130223B: GTEX Patient 10B Nuclei combined aliquots | ||
* RNA extracted using Zymo micro RNA kit | * RNA extracted using Zymo micro RNA kit (eluted in 6ul) | ||
RNA Concentrations: | |||
# Naac Bulk sort - 18.2ng/ul | |||
# GTEX 10A Bulk Sort (combined 5 aliquots) - 5.17ng/ul | |||
# GTEX 10B Bulk Sort (combined 5 aliquots) - 5.10ng/ul | |||
Ran Bioanalyzer using Pico chip and comparing to BA8 sort pool RNA ([[http://genome-tech.ucsd.edu/LabNotes/index.php/Blue:RNA-Seq_Experiments:04302014]]: | |||
[[media:RNA_Pico_2015-03-06_14-36-22.pdf]] |
Revision as of 01:10, 7 March 2015
C1 Tube Controls
- Testing the quality of RNA for the following C1 runs:
- 20150205A: Naac bulk sorted nuclei (MSSM)
- 20130223A: GTEX Patient 10A Nuclei combined aliquots
- 20130223B: GTEX Patient 10B Nuclei combined aliquots
- RNA extracted using Zymo micro RNA kit (eluted in 6ul)
RNA Concentrations:
- Naac Bulk sort - 18.2ng/ul
- GTEX 10A Bulk Sort (combined 5 aliquots) - 5.17ng/ul
- GTEX 10B Bulk Sort (combined 5 aliquots) - 5.10ng/ul
Ran Bioanalyzer using Pico chip and comparing to BA8 sort pool RNA ([[1]]: