Blue:RNA-Seq Experiments:03062015: Difference between revisions
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# GTEX 10B Bulk Sort (combined 5 aliquots) - 5.10ng/ul | # GTEX 10B Bulk Sort (combined 5 aliquots) - 5.10ng/ul | ||
Diluted RNA 1:10 and ran on Bioanalyzer using Pico chip: | |||
*Blue1 = Naac RNA | |||
*Blue2 = GTEX 10A RNA | |||
*Blue3 = GTEX 10B RNA | |||
*Blue4 = BA8 sort pool RNA ([[http://genome-tech.ucsd.edu/LabNotes/index.php/Blue:RNA-Seq_Experiments:04302014]] | |||
[[media:RNA_Pico_2015-03-06_14-36-22.pdf]] | [[media:RNA_Pico_2015-03-06_14-36-22.pdf]] |
Latest revision as of 01:12, 7 March 2015
C1 Tube Controls[edit]
- Testing the quality of RNA for the following C1 runs:
- 20150205A: Naac bulk sorted nuclei (MSSM)
- 20130223A: GTEX Patient 10A Nuclei combined aliquots
- 20130223B: GTEX Patient 10B Nuclei combined aliquots
- RNA extracted using Zymo micro RNA kit (eluted in 6ul)
RNA Concentrations:
- Naac Bulk sort - 18.2ng/ul
- GTEX 10A Bulk Sort (combined 5 aliquots) - 5.17ng/ul
- GTEX 10B Bulk Sort (combined 5 aliquots) - 5.10ng/ul
Diluted RNA 1:10 and ran on Bioanalyzer using Pico chip:
- Blue1 = Naac RNA
- Blue2 = GTEX 10A RNA
- Blue3 = GTEX 10B RNA
- Blue4 = BA8 sort pool RNA ([[1]]