Matt:LabNotes/2015-6-15: Difference between revisions

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| align="center" style="background:#f0f0f0;"|'''Stdev'''
| align="center" style="background:#f0f0f0;"|'''Stdev'''
|-
|-
| KIT 1:1||1228||1228||||
| KIT 1:1||1053.5||1053.5||||
|-
|-
| KIT 1:5||271.8||1359||||
| KIT 1:5||212.5||1062.5||||
|-
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| KIT 1:10||135.4||1354||1313.7||74.2
| KIT 1:10||104.9||1049||1055||6.87
|-
|-
| SNAP25 1:1||1227.6||1227.6||||
| SNAP25 1:1||1039.5||1039.5||||
|-
|-
| SNAP25 1:5||258.5||1292.5||||
| SNAP25 1:5||207.7||1038.5||||
|-
|-
| SNAP25 1:10||128.8||1288||1269.4||36.2
| SNAP25 1:10||107.4||1074||1050.67||20.21
|-
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|}

Revision as of 01:36, 16 June 2015

Motor Neurons RNA FISH Dye Coupling

Probe Resuspension

  1. Resuspend each oligo in 15 uL nuclase-free H2O (1 mM)
  2. Take 2 uL from each oligo well and combine them in a single 1.5 mL tube (total concentration 1 mM)
    1. Store the remaining oligos at -20C
  3. Add 450 uL nuclase-free, diluting 10:1, for final concentration of 100 uM
    • Technically closer to 5:1 dilution but final result is closer to 10:1 because starting concentration is actually lower...
    • Goal is to get 1ug/ul DNA concentration
  4. Check concentration in nanodrop at 1:1, 5:1, and 10:1 dilutions for accuracy