Andrew:Notebook/AR150702 C1: Difference between revisions
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====Sequencing==== | ====Sequencing==== | ||
First round of sequencing | |||
{| {{table}} | {| {{table}} | ||
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| AR-150702-G01-Tn5059-N701-S508||TAAGGCGA-CTAAGCCT||3||SE50+8+8||200-800||HP10||HP12||NA||DT Tn5 New | | AR-150702-G01-Tn5059-N701-S508||TAAGGCGA-CTAAGCCT||3||SE50+8+8||200-800||HP10||HP12||NA||DT Tn5 New | ||
|- | |||
| | |||
|} | |||
2nd Round of sequencing | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''SampleID''' | |||
| align="center" style="background:#f0f0f0;"|'''Index Sequence''' | |||
| align="center" style="background:#f0f0f0;"|'''# of Reads (M)''' | |||
| align="center" style="background:#f0f0f0;"|'''Run Type, Minmum BP''' | |||
| align="center" style="background:#f0f0f0;"|'''Size range (bp)''' | |||
| align="center" style="background:#f0f0f0;"|'''Read 1 Primer''' | |||
| align="center" style="background:#f0f0f0;"|'''Index 1 Primer''' | |||
| align="center" style="background:#f0f0f0;"|'''Read 2 Primer''' | |||
| align="center" style="background:#f0f0f0;"|'''Tube labeled''' | |||
|- | |||
| AR-150702-Ch03-A01-N703-S502||AGGCAGAA-CTCTCTAT||2||SE50+8+8||200-800||HP10||HP12||NA||AR150714_Pool1 | |||
|- | |||
| AR-150702-Ch09-B01-N703-S503||AGGCAGAA-TATCCTCT||2||SE50+8+8||200-800||HP10||HP12||NA||AR150714_Pool1 | |||
|- | |||
| AR-150702-Ch22-D09-N703-S505||AGGCAGAA-GTAAGGAG||2||SE50+8+8||200-800||HP10||HP12||NA||AR150714_Pool1 | |||
|- | |||
| AR-150702-Ch44-H02-N703-S506||AGGCAGAA-ACTGCATA||2||SE50+8+8||200-800||HP10||HP12||NA||AR150714_Pool1 | |||
|- | |||
| AR-150702-Ch45-H03-N703-S507||AGGCAGAA-AAGGAGTA||2||SE50+8+8||200-800||HP10||HP12||NA||AR150714_Pool2 | |||
|- | |||
| AR-150702-Ch46-H07-N703-S508||AGGCAGAA-CTAAGCCT||2||SE50+8+8||200-800||HP10||HP12||NA||AR150714_Pool2 | |||
|- | |||
| AR-150702-Ch47-H08-N703-S504||AGGCAGAA-AGAGTAGA||2||SE50+8+8||200-800||HP10||HP12||NA||AR150714_Pool2 | |||
|- | |||
| AR-150702-Ch48-H09-N703-S517||AGGCAGAA-GCGTAAGA||2||SE50+8+8||200-800||HP10||HP12||NA||AR150714_Pool2 | |||
|- | |||
| AR-150702-D01-NxtXT-N702-S502||CGTACTAG-CTCTCTAT||0.67||SE50+8+8||200-800||HP10||HP12||NA||DT Tn5 Old | |||
|- | |||
| AR-150702-E01-NxtXT-N702-S503||CGTACTAG-TATCCTCT||0.67||SE50+8+8||200-800||HP10||HP12||NA||DT Tn5 Old | |||
|- | |||
| AR-150702-G01-NxtXT-N702-S505||CGTACTAG-GTAAGGAG||0.67||SE50+8+8||200-800||HP10||HP12||NA||DT Tn5 Old | |||
|- | |||
| AR-150702-D01-CoRE-NEBNext25||TACTGC||0.67||SE50+8+8||200-800||HP10||HP12||NA||DT Tn5 Old | |||
|- | |||
| AR-150702-E01-CoRE-NEBNext26||TCGTGA||0.67||SE50+8+8||200-800||HP10||HP12||NA||DT Tn5 Old | |||
|- | |||
| AR-150702-G01-CoRE-NEBNext27||TACGCG||0.67||SE50+8+8||200-800||HP10||HP12||NA||DT Tn5 Old | |||
|- | |- | ||
| | | | ||
|} | |} |
Revision as of 19:41, 14 July 2015
Sequential MDA on C1 with PEO1
- July 2, 2015
- Experiment ID: AR150702_C1
Results
Gels
File:AR150702 C1 gelsSlide1.png
File:AR150702 C1 gelsSlide2.png
File:AR150702 C1 gelsSlide3.png
File:AR150702 C1 gelsSlide4.png
Sequencing
First round of sequencing
Sample ID | Index Sequence | # of Reads | Run Type, Minmum BP | Library Size | Read 1 Sequencing Primer | Index Primer | Read 2 Sequencing Primer | Notes |
INITIAL-PROJECT-SAMPLE-DATE-Index | CGTGCA | Million | SE/PE, 36+6+36 | SIZE RANGE (BP) | Name | Name | Name | |
AR-150702-D01-NxtXT-N702-S502 | CGTACTAG-CTCTCTAT | 1.5 | SE50+8+8 | 200-800 | HP10 | HP12 | NA | DT Tn5 Old |
AR-150702-E01-NxtXT-N702-S503 | CGTACTAG-TATCCTCT | 1.5 | SE50+8+8 | 200-800 | HP10 | HP12 | NA | DT Tn5 Old |
AR-150702-G01-NxtXT-N702-S505 | CGTACTAG-GTAAGGAG | 1.5 | SE50+8+8 | 200-800 | HP10 | HP12 | NA | DT Tn5 Old |
AR-150702-D01-CoRE-NEBNext25 | TACTGC | 1.5 | SE50+8+8 | 200-800 | HP10 | HP12 | NA | DT Tn5 Old |
AR-150702-E01-CoRE-NEBNext26 | TCGTGA | 1.5 | SE50+8+8 | 200-800 | HP10 | HP12 | NA | DT Tn5 Old |
AR-150702-G01-CoRE-NEBNext27 | TACGCG | 1.5 | SE50+8+8 | 200-800 | HP10 | HP12 | NA | DT Tn5 Old |
AR-150702-D01-Tn5059-N701-S506 | TAAGGCGA-ACTGCATA | 3 | SE50+8+8 | 200-800 | HP10 | HP12 | NA | DT Tn5 New |
AR-150702-E01-Tn5059-N701-S507 | TAAGGCGA-AAGGAGTA | 3 | SE50+8+8 | 200-800 | HP10 | HP12 | NA | DT Tn5 New |
AR-150702-G01-Tn5059-N701-S508 | TAAGGCGA-CTAAGCCT | 3 | SE50+8+8 | 200-800 | HP10 | HP12 | NA | DT Tn5 New |
2nd Round of sequencing
SampleID | Index Sequence | # of Reads (M) | Run Type, Minmum BP | Size range (bp) | Read 1 Primer | Index 1 Primer | Read 2 Primer | Tube labeled |
AR-150702-Ch03-A01-N703-S502 | AGGCAGAA-CTCTCTAT | 2 | SE50+8+8 | 200-800 | HP10 | HP12 | NA | AR150714_Pool1 |
AR-150702-Ch09-B01-N703-S503 | AGGCAGAA-TATCCTCT | 2 | SE50+8+8 | 200-800 | HP10 | HP12 | NA | AR150714_Pool1 |
AR-150702-Ch22-D09-N703-S505 | AGGCAGAA-GTAAGGAG | 2 | SE50+8+8 | 200-800 | HP10 | HP12 | NA | AR150714_Pool1 |
AR-150702-Ch44-H02-N703-S506 | AGGCAGAA-ACTGCATA | 2 | SE50+8+8 | 200-800 | HP10 | HP12 | NA | AR150714_Pool1 |
AR-150702-Ch45-H03-N703-S507 | AGGCAGAA-AAGGAGTA | 2 | SE50+8+8 | 200-800 | HP10 | HP12 | NA | AR150714_Pool2 |
AR-150702-Ch46-H07-N703-S508 | AGGCAGAA-CTAAGCCT | 2 | SE50+8+8 | 200-800 | HP10 | HP12 | NA | AR150714_Pool2 |
AR-150702-Ch47-H08-N703-S504 | AGGCAGAA-AGAGTAGA | 2 | SE50+8+8 | 200-800 | HP10 | HP12 | NA | AR150714_Pool2 |
AR-150702-Ch48-H09-N703-S517 | AGGCAGAA-GCGTAAGA | 2 | SE50+8+8 | 200-800 | HP10 | HP12 | NA | AR150714_Pool2 |
AR-150702-D01-NxtXT-N702-S502 | CGTACTAG-CTCTCTAT | 0.67 | SE50+8+8 | 200-800 | HP10 | HP12 | NA | DT Tn5 Old |
AR-150702-E01-NxtXT-N702-S503 | CGTACTAG-TATCCTCT | 0.67 | SE50+8+8 | 200-800 | HP10 | HP12 | NA | DT Tn5 Old |
AR-150702-G01-NxtXT-N702-S505 | CGTACTAG-GTAAGGAG | 0.67 | SE50+8+8 | 200-800 | HP10 | HP12 | NA | DT Tn5 Old |
AR-150702-D01-CoRE-NEBNext25 | TACTGC | 0.67 | SE50+8+8 | 200-800 | HP10 | HP12 | NA | DT Tn5 Old |
AR-150702-E01-CoRE-NEBNext26 | TCGTGA | 0.67 | SE50+8+8 | 200-800 | HP10 | HP12 | NA | DT Tn5 Old |
AR-150702-G01-CoRE-NEBNext27 | TACGCG | 0.67 | SE50+8+8 | 200-800 | HP10 | HP12 | NA | DT Tn5 Old |