Andrew:Notebook/AR150702 C1: Difference between revisions

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====Sequencing====
====Sequencing====
First round of sequencing


{| {{table}}
{| {{table}}
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| AR-150702-G01-Tn5059-N701-S508||TAAGGCGA-CTAAGCCT||3||SE50+8+8||200-800||HP10||HP12||NA||DT Tn5 New
| AR-150702-G01-Tn5059-N701-S508||TAAGGCGA-CTAAGCCT||3||SE50+8+8||200-800||HP10||HP12||NA||DT Tn5 New
|-
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|}
2nd Round of sequencing
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''SampleID'''
| align="center" style="background:#f0f0f0;"|'''Index Sequence'''
| align="center" style="background:#f0f0f0;"|'''# of Reads (M)'''
| align="center" style="background:#f0f0f0;"|'''Run Type, Minmum BP'''
| align="center" style="background:#f0f0f0;"|'''Size range (bp)'''
| align="center" style="background:#f0f0f0;"|'''Read 1 Primer'''
| align="center" style="background:#f0f0f0;"|'''Index 1 Primer'''
| align="center" style="background:#f0f0f0;"|'''Read 2 Primer'''
| align="center" style="background:#f0f0f0;"|'''Tube labeled'''
|-
| AR-150702-Ch03-A01-N703-S502||AGGCAGAA-CTCTCTAT||2||SE50+8+8||200-800||HP10||HP12||NA||AR150714_Pool1
|-
| AR-150702-Ch09-B01-N703-S503||AGGCAGAA-TATCCTCT||2||SE50+8+8||200-800||HP10||HP12||NA||AR150714_Pool1
|-
| AR-150702-Ch22-D09-N703-S505||AGGCAGAA-GTAAGGAG||2||SE50+8+8||200-800||HP10||HP12||NA||AR150714_Pool1
|-
| AR-150702-Ch44-H02-N703-S506||AGGCAGAA-ACTGCATA||2||SE50+8+8||200-800||HP10||HP12||NA||AR150714_Pool1
|-
| AR-150702-Ch45-H03-N703-S507||AGGCAGAA-AAGGAGTA||2||SE50+8+8||200-800||HP10||HP12||NA||AR150714_Pool2
|-
| AR-150702-Ch46-H07-N703-S508||AGGCAGAA-CTAAGCCT||2||SE50+8+8||200-800||HP10||HP12||NA||AR150714_Pool2
|-
| AR-150702-Ch47-H08-N703-S504||AGGCAGAA-AGAGTAGA||2||SE50+8+8||200-800||HP10||HP12||NA||AR150714_Pool2
|-
| AR-150702-Ch48-H09-N703-S517||AGGCAGAA-GCGTAAGA||2||SE50+8+8||200-800||HP10||HP12||NA||AR150714_Pool2
|-
| AR-150702-D01-NxtXT-N702-S502||CGTACTAG-CTCTCTAT||0.67||SE50+8+8||200-800||HP10||HP12||NA||DT Tn5 Old
|-
| AR-150702-E01-NxtXT-N702-S503||CGTACTAG-TATCCTCT||0.67||SE50+8+8||200-800||HP10||HP12||NA||DT Tn5 Old
|-
| AR-150702-G01-NxtXT-N702-S505||CGTACTAG-GTAAGGAG||0.67||SE50+8+8||200-800||HP10||HP12||NA||DT Tn5 Old
|-
| AR-150702-D01-CoRE-NEBNext25||TACTGC||0.67||SE50+8+8||200-800||HP10||HP12||NA||DT Tn5 Old
|-
| AR-150702-E01-CoRE-NEBNext26||TCGTGA||0.67||SE50+8+8||200-800||HP10||HP12||NA||DT Tn5 Old
|-
| AR-150702-G01-CoRE-NEBNext27||TACGCG||0.67||SE50+8+8||200-800||HP10||HP12||NA||DT Tn5 Old
|-
|-
|  
|  
|}
|}

Revision as of 19:41, 14 July 2015

Sequential MDA on C1 with PEO1

  • July 2, 2015
  • Experiment ID: AR150702_C1

Results

Gels

File:AR150702 C1 gelsSlide1.png

File:AR150702 C1 gelsSlide2.png

File:AR150702 C1 gelsSlide3.png

File:AR150702 C1 gelsSlide4.png

Sequencing

First round of sequencing

Sample ID Index Sequence # of Reads Run Type, Minmum BP Library Size Read 1 Sequencing Primer Index Primer Read 2 Sequencing Primer Notes
INITIAL-PROJECT-SAMPLE-DATE-Index CGTGCA Million SE/PE, 36+6+36 SIZE RANGE (BP) Name Name Name
AR-150702-D01-NxtXT-N702-S502 CGTACTAG-CTCTCTAT 1.5 SE50+8+8 200-800 HP10 HP12 NA DT Tn5 Old
AR-150702-E01-NxtXT-N702-S503 CGTACTAG-TATCCTCT 1.5 SE50+8+8 200-800 HP10 HP12 NA DT Tn5 Old
AR-150702-G01-NxtXT-N702-S505 CGTACTAG-GTAAGGAG 1.5 SE50+8+8 200-800 HP10 HP12 NA DT Tn5 Old
AR-150702-D01-CoRE-NEBNext25 TACTGC 1.5 SE50+8+8 200-800 HP10 HP12 NA DT Tn5 Old
AR-150702-E01-CoRE-NEBNext26 TCGTGA 1.5 SE50+8+8 200-800 HP10 HP12 NA DT Tn5 Old
AR-150702-G01-CoRE-NEBNext27 TACGCG 1.5 SE50+8+8 200-800 HP10 HP12 NA DT Tn5 Old
AR-150702-D01-Tn5059-N701-S506 TAAGGCGA-ACTGCATA 3 SE50+8+8 200-800 HP10 HP12 NA DT Tn5 New
AR-150702-E01-Tn5059-N701-S507 TAAGGCGA-AAGGAGTA 3 SE50+8+8 200-800 HP10 HP12 NA DT Tn5 New
AR-150702-G01-Tn5059-N701-S508 TAAGGCGA-CTAAGCCT 3 SE50+8+8 200-800 HP10 HP12 NA DT Tn5 New

2nd Round of sequencing

SampleID Index Sequence # of Reads (M) Run Type, Minmum BP Size range (bp) Read 1 Primer Index 1 Primer Read 2 Primer Tube labeled
AR-150702-Ch03-A01-N703-S502 AGGCAGAA-CTCTCTAT 2 SE50+8+8 200-800 HP10 HP12 NA AR150714_Pool1
AR-150702-Ch09-B01-N703-S503 AGGCAGAA-TATCCTCT 2 SE50+8+8 200-800 HP10 HP12 NA AR150714_Pool1
AR-150702-Ch22-D09-N703-S505 AGGCAGAA-GTAAGGAG 2 SE50+8+8 200-800 HP10 HP12 NA AR150714_Pool1
AR-150702-Ch44-H02-N703-S506 AGGCAGAA-ACTGCATA 2 SE50+8+8 200-800 HP10 HP12 NA AR150714_Pool1
AR-150702-Ch45-H03-N703-S507 AGGCAGAA-AAGGAGTA 2 SE50+8+8 200-800 HP10 HP12 NA AR150714_Pool2
AR-150702-Ch46-H07-N703-S508 AGGCAGAA-CTAAGCCT 2 SE50+8+8 200-800 HP10 HP12 NA AR150714_Pool2
AR-150702-Ch47-H08-N703-S504 AGGCAGAA-AGAGTAGA 2 SE50+8+8 200-800 HP10 HP12 NA AR150714_Pool2
AR-150702-Ch48-H09-N703-S517 AGGCAGAA-GCGTAAGA 2 SE50+8+8 200-800 HP10 HP12 NA AR150714_Pool2
AR-150702-D01-NxtXT-N702-S502 CGTACTAG-CTCTCTAT 0.67 SE50+8+8 200-800 HP10 HP12 NA DT Tn5 Old
AR-150702-E01-NxtXT-N702-S503 CGTACTAG-TATCCTCT 0.67 SE50+8+8 200-800 HP10 HP12 NA DT Tn5 Old
AR-150702-G01-NxtXT-N702-S505 CGTACTAG-GTAAGGAG 0.67 SE50+8+8 200-800 HP10 HP12 NA DT Tn5 Old
AR-150702-D01-CoRE-NEBNext25 TACTGC 0.67 SE50+8+8 200-800 HP10 HP12 NA DT Tn5 Old
AR-150702-E01-CoRE-NEBNext26 TCGTGA 0.67 SE50+8+8 200-800 HP10 HP12 NA DT Tn5 Old
AR-150702-G01-CoRE-NEBNext27 TACGCG 0.67 SE50+8+8 200-800 HP10 HP12 NA DT Tn5 Old