Andrew:Notebook/AR150729 inTube: Difference between revisions

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==Prep==
===Motivation===
Sequential MDA on the C1 with unmodified primer failed on 7/20. We suspect that the unmodified N9 was digested too quickly, leading to low or zero efficiency for the first MDA reaction. This can be tested in tube.
===Protocol===
[[File:AR150729_reagents-page-001.jpg|600px]]
[[File:AR150729_reagents-page-001.jpg|600px]]
==Results==
===Gels===
===Sequencing===
[[File:AR150729_inTube-page-001.jpg|600px]]

Revision as of 17:51, 11 September 2015

Prep

Motivation

Sequential MDA on the C1 with unmodified primer failed on 7/20. We suspect that the unmodified N9 was digested too quickly, leading to low or zero efficiency for the first MDA reaction. This can be tested in tube.

Protocol

File:AR150729 reagents-page-001.jpg

Results

Gels

Sequencing

File:AR150729 inTube-page-001.jpg