Andrew:Notebook/AR150729 inTube: Difference between revisions
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==Prep== | |||
===Motivation=== | |||
Sequential MDA on the C1 with unmodified primer failed on 7/20. We suspect that the unmodified N9 was digested too quickly, leading to low or zero efficiency for the first MDA reaction. This can be tested in tube. | |||
===Protocol=== | |||
[[File:AR150729_reagents-page-001.jpg|600px]] | [[File:AR150729_reagents-page-001.jpg|600px]] | ||
==Results== | |||
===Gels=== | |||
===Sequencing=== | |||
[[File:AR150729_inTube-page-001.jpg|600px]] |
Revision as of 17:51, 11 September 2015
Prep
Motivation
Sequential MDA on the C1 with unmodified primer failed on 7/20. We suspect that the unmodified N9 was digested too quickly, leading to low or zero efficiency for the first MDA reaction. This can be tested in tube.
Protocol
File:AR150729 reagents-page-001.jpg