Daniel:Notebook/RNAFACS/2015-10-30: Difference between revisions
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>Djacobse No edit summary |
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File:20151030.lif_Series001Snapshot1_ch00.jpg|Field 1 | File:20151030.lif_Series001Snapshot1_ch00.jpg|Field 1 | ||
File:20151030.lif_Series003Snapshot1_ch00.jpg|Field 2 | File:20151030.lif_Series003Snapshot1_ch00.jpg|Field 2 | ||
File:20151030.lif_Series004Snapshot1_ch00.jpg|Field 3 | File:20151030.lif_Series004Snapshot1_ch00.jpg|Field 3; several nuclei in this frame, only 1 shows both FEZF2 and RBFOX3 signal | ||
</gallery> | </gallery> | ||
[[Category:RNAFACS]] [[Category:RNAScope]] [[Category:RNAScope-10X-I]] | [[Category:RNAFACS]] [[Category:RNAScope]] [[Category:RNAScope-10X-I]] |
Revision as of 16:11, 30 October 2015
RNA Scope Trial Run 5: 10X-I
Microcscopy Results
So I tried to look at the results under the 10X. Unfortunately, I check and DAPI quenches DRAQ5. There is a lot of signal under the DAPI channel, but I cannot visualize the nuclei, which makes it very difficult to take images.
- Taken with 63X oil on confocal; max z-plane; Red=Draq5 (C2), Green=FEZF2 (C3); Yellow=RBFOX3/NeuN (C1)
- 20151030.lif Series001Snapshot1 ch00.jpg
Field 1
- 20151030.lif Series003Snapshot1 ch00.jpg
Field 2
- 20151030.lif Series004Snapshot1 ch00.jpg
Field 3; several nuclei in this frame, only 1 shows both FEZF2 and RBFOX3 signal