Daniel:Notebook/RNAFACS/2015-10-30: Difference between revisions
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==Microcscopy Results== | ==Microcscopy Results== | ||
So I tried to look at the results under the 10X. Unfortunately, I | So I tried to look at the results under the 10X. Unfortunately, I [http://www.abcam.com/ps/products/108/ab108410/documents/ab108410%20DRAQ5%20v2%20(Web).pdf checked] and DAPI quenches DRAQ5. There is a lot of signal under the DAPI channel, but I cannot visualize the nuclei, which makes it very difficult to take images. | ||
<gallery heights=300px widths=300px perrow=3 caption="Taken with 63X oil on confocal; max z-plane; Red=Draq5 (C2), Green=FEZF2 (C3); Yellow=RBFOX3/NeuN (C1)"> | <gallery heights=300px widths=300px perrow=3 caption="Taken with 63X oil on confocal; max z-plane; Red=Draq5 (C2), Green=FEZF2 (C3); Yellow=RBFOX3/NeuN (C1)"> |
Latest revision as of 16:12, 30 October 2015
RNA Scope Trial Run 5: 10X-I[edit]
Microcscopy Results[edit]
So I tried to look at the results under the 10X. Unfortunately, I checked and DAPI quenches DRAQ5. There is a lot of signal under the DAPI channel, but I cannot visualize the nuclei, which makes it very difficult to take images.
- 20151030.lif Series001Snapshot1 ch00.jpg
Field 1
- 20151030.lif Series003Snapshot1 ch00.jpg
Field 2
- 20151030.lif Series004Snapshot1 ch00.jpg
Field 3; several nuclei in this frame, only 1 shows both FEZF2 and RBFOX3 signal