Daniel:Notebook/ComboLock/2016-3-15: Difference between revisions

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(Created page with "=10% Acrylamide VA-044= Back to Calendar From yesterday's results we decided to increase the acrylamide...")
 
>Djacobse
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| align="center" align="center" valign="bottom" | 40%
| align="center" align="center" valign="bottom" | 40%
| align="center" align="center" valign="bottom" | 9%
| align="center" align="center" valign="bottom" | 9%
| align="center" valign="bottom" | 9 mL
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| align="center" valign="bottom" | 40% (38:2)
| align="center" valign="bottom" | 40% (38:2)
| align="center" valign="bottom" | 0.95:0.05 (%)
| align="center" valign="bottom" | 0.95:0.05 (%)
| align="center" valign="bottom" | 1 mL
| align="center" valign="bottom" | 0.75 mL


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| align="center" valign="bottom" | -----
| align="center" valign="bottom" | -----
| align="center" valign="bottom" | 0.25% (m/v)
| align="center" valign="bottom" | 0.25% (m/v)
| align="center" valign="bottom" | 0.1 g
| align="center" valign="bottom" | 0.075 g


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| align="center" valign="bottom" | 10X
| align="center" valign="bottom" | 10X
| align="center" valign="bottom" | 1X
| align="center" valign="bottom" | 1X
| align="center" valign="bottom" | 4 mL
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| align="center" valign="bottom" | 26 mL
| align="center" valign="bottom" | 9.5 mL


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|style="font-weight:bold" align="center" valign="bottom" | 40 mL
|style="font-weight:bold" align="center" valign="bottom" | 30 mL


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Revision as of 21:32, 15 March 2016

10% Acrylamide VA-044

Back to Calendar

From yesterday's results we decided to increase the acrylamide concentration in the gel solution in order to make up for a lack of formaldehyde/tissue.

VA-044/10% Acrylamide test

For test 2 we used the same setup as test 1, but with the following recipe for the VA-044 gel mix:

Reagent Stock Concentration Final Concentration Amount added
Acrylamide 40% 9% 6.75 mL
Acrylamide:Bis (19:1) 40% (38:2) 0.95:0.05 (%) 0.75 mL
VA-044 ----- 0.25% (m/v) 0.075 g
PBS 10X 1X 3 mL
ddH2O ----- ----- 9.5 mL
Total     30 mL


Run Parameters

  • Line 1: Oil (on ice); X psi; ~2 mL
  • Line 2: Aqueous (VA-044P; on ice); X psi; ~1 mL

Results