Daniel:Notebook/ComboLock/CProbeDesign: Difference between revisions
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>Djacobse (Created page with "=C Probe Design= Back to Notebook This page describes the methods used for designing C-Probes for Combo Lock. The illustration below shows wha...") |
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[[Image:C_Probes.png|600px]] | [[Image:C_Probes.png|600px]] | ||
==Designing Transcript Matching Region== | |||
*Taken from [http://www.nature.com/nmeth/journal/v13/n3/abs/nmeth.3742.html Frei et al paper] | |||
*[https://github.com/nolanlab/PLAYRDesign/blob/master/README.md README] for probe design software | |||
#Must install R, Primer3, and Blast+ before using | |||
#Make blast data | |||
#In R: | |||
library(devtools) | |||
install_github("nolanlab/PLAYRDesign") | |||
library(PLAYRDesign) |
Revision as of 16:08, 21 April 2016
C Probe Design
This page describes the methods used for designing C-Probes for Combo Lock. The illustration below shows what a C-probe is expected to look like. The descriptions given include the original design sizes.
Designing Transcript Matching Region
- Taken from Frei et al paper
- README for probe design software
- Must install R, Primer3, and Blast+ before using
- Make blast data
- In R:
library(devtools) install_github("nolanlab/PLAYRDesign") library(PLAYRDesign)