Daniel:Protocols/AbHybridization: Difference between revisions

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>Djacobse
>Djacobse
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<li>Centrifuge samples and incubate overnight at 4C</li>
<li>Centrifuge samples and incubate overnight at 4C</li>
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==PLA Paper==
[http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0025583#s4 This paper] is actually also by Darmanis.  It covers a different style of PEA analysis.

Revision as of 23:17, 6 May 2016

Antibody Hybridization

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This page details the protocol from Darmanis et al paper. The protocol is for hybridizing antibodies linked with oligonucleotides to their protein targets. The protocol will be used in validation experiments for Combo-Lock.

Darmanis Protocol

  1. Mix 1 uL cell lysate with 3 uL antibody mix
  2. Antibody Mix Contains
    1. 0.3 uL Probe A
    2. 0.3 uL Probe B
    3. 0.2 uL Incubation Stabilizer (Olink Bioscience)
    4. 2.1 uL Incubation Solution (Olink Biosicence)
  3. Centrifuge samples and incubate overnight at 4C

PLA Paper

This paper is actually also by Darmanis. It covers a different style of PEA analysis.