Alice:LabNotes/2007-11-26: Difference between revisions
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Since the agrose gel on 11/16/07 didn't work out as expected, therefore I ran another gel with the same procedure. Each well contain total of 5 ul of mixture of reagents.<br> | |||
<b>Summary of volumes of Reagents used:</b> | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''well 1''' | |||
| align="center" style="background:#f0f0f0;"|'''well 2''' | |||
| align="center" style="background:#f0f0f0;"|'''well 3''' | |||
| align="center" style="background:#f0f0f0;"|'''well 4''' | |||
| align="center" style="background:#f0f0f0;"|'''well 5''' | |||
| align="center" style="background:#f0f0f0;"|'''well 6''' | |||
| align="center" style="background:#f0f0f0;"|'''well 7''' | |||
| align="center" style="background:#f0f0f0;"|'''well 8''' | |||
|- | |||
| Low Mass bp Ladder ||3 ul ||0 ul ||0 ul||0 ul||0 ul||0 ul||0 ul||0 ul|| | |||
|- | |||
| 100 bp Ladder ||0 ul ||3 ul ||0 ul||0 ul||0 ul||0 ul||0 ul||0 ul|| | |||
|- | |||
| TBE buffer || 1 ul|| 1 ul|| 0 ul||0 ul||0 ul||0 ul||0 ul||0 ul|| | |||
|- | |||
| Novex 5x TBE loading dye || 1 ul|| 1 ul|| 1 ul||1 ul||1 ul||1 ul||1 ul||1 ul|| | |||
|- | |||
| post-PCR sample || 0 ul|| 0 ul|| 4 ul||4 ul||4 ul||4 ul||4 ul||4 ul|| | |||
|- | |||
| | |||
|} | |||
<b>Results:</b><br> | |||
[[Image:ZhangLab_2 2007-11-26 13hr 17min-2-.png]] <br> | [[Image:ZhangLab_2 2007-11-26 13hr 17min-2-.png]] <br> | ||
First of all, the intensity of the gel was not bright enough. This indicates that either the gel didn't get stained long enough or the staining solution has gone bad. But the second condition was very unlikely since professor Zhang ran some very good gel with the same staining solution. Also, the focus of the gel imaging machine could be adjusted to be either further away of closer to the level of samples, since the image shows some fuzzy edges. | |||
[[Image:ZhangLab_2 2007-11-26 14hr 29min.png]]<br> | [[Image:ZhangLab_2 2007-11-26 14hr 29min.png]]<br> | ||
[[Image:ZhangLab_2 2007-11-26 14hr 36min.png]] | [[Image:ZhangLab_2 2007-11-26 14hr 36min.png]] |
Revision as of 07:19, 2 December 2007
Since the agrose gel on 11/16/07 didn't work out as expected, therefore I ran another gel with the same procedure. Each well contain total of 5 ul of mixture of reagents.
Summary of volumes of Reagents used:
' | well 1 | well 2 | well 3 | well 4 | well 5 | well 6 | well 7 | well 8 | |
Low Mass bp Ladder | 3 ul | 0 ul | 0 ul | 0 ul | 0 ul | 0 ul | 0 ul | 0 ul | |
100 bp Ladder | 0 ul | 3 ul | 0 ul | 0 ul | 0 ul | 0 ul | 0 ul | 0 ul | |
TBE buffer | 1 ul | 1 ul | 0 ul | 0 ul | 0 ul | 0 ul | 0 ul | 0 ul | |
Novex 5x TBE loading dye | 1 ul | 1 ul | 1 ul | 1 ul | 1 ul | 1 ul | 1 ul | 1 ul | |
post-PCR sample | 0 ul | 0 ul | 4 ul | 4 ul | 4 ul | 4 ul | 4 ul | 4 ul | |
Results:
File:ZhangLab 2 2007-11-26 13hr 17min-2-.png
First of all, the intensity of the gel was not bright enough. This indicates that either the gel didn't get stained long enough or the staining solution has gone bad. But the second condition was very unlikely since professor Zhang ran some very good gel with the same staining solution. Also, the focus of the gel imaging machine could be adjusted to be either further away of closer to the level of samples, since the image shows some fuzzy edges.
File:ZhangLab 2 2007-11-26 14hr 29min.png
File:ZhangLab 2 2007-11-26 14hr 36min.png