Daniel:Protocols/LatchPadlock: Difference between revisions
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<li>After washing, incubate with 100 nM insert/backbone oligos for 30 min at 37C</li> | <li>After washing, incubate with 100 nM insert/backbone oligos for 30 min at 37C</li> | ||
<li>Wash twice</li> | <li>Wash twice</li> | ||
<ol type="A"><li>Wash by pelleting cells at 600xg for 3 min</li></ol> | |||
</ol> | </ol> |
Revision as of 23:44, 8 June 2016
Latch and Padlock Hybridization
Protocol
- Start from hybridized C Probes or antibodies
- Make sure probes have been washed as per the protocol
- After washing, incubate with 100 nM insert/backbone oligos for 30 min at 37C
- Wash twice
- Wash by pelleting cells at 600xg for 3 min
Buffers
- Insert/backbone Hybridization Buffer
- 100 nM insert/backbone oligos
- 1X SSC
- 40 U/mL RNasin
- PBS
- Wash Buffer
- PBS
- 0.1% Tween
- 4 U/mL RNasin