Daniel:Notebook/ComboLock/Sequencing: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Djacobse
>Djacobse
Line 94: Line 94:
| width="127" | Indexes
| width="127" | Indexes
| width="300" | Index Sequences
| width="300" | Index Sequences
| width="150" | Results


|- style="font-size:12pt"
|- style="font-size:12pt"
Line 100: Line 101:
|style="text-decoration:none" align="center" align="center" | 2,3,5,6
|style="text-decoration:none" align="center" align="center" | 2,3,5,6
|  | CGATGT, TTAGGC, ACAGTG, GCCAAT
|  | CGATGT, TTAGGC, ACAGTG, GCCAAT
| &nbsp


|- style="background-color:#D9D9D9;font-size:12pt"
|- style="background-color:#D9D9D9;font-size:12pt"
Line 106: Line 108:
|style="text-decoration:none" align="center" | 1-7
|style="text-decoration:none" align="center" | 1-7
|  | ATCACG, CGATGT, TTAGGC, TGACCA, ACAGTG, GCCAAT, CAGATC
|  | ATCACG, CGATGT, TTAGGC, TGACCA, ACAGTG, GCCAAT, CAGATC
| &nbsp


|- style="font-size:12pt"
|- style="font-size:12pt"
Line 112: Line 115:
|style="text-decoration:none" align="center" | 10-13
|style="text-decoration:none" align="center" | 10-13
|  | TAGCTT, GGCTAC, CTTGTA, CCATGA
|  | TAGCTT, GGCTAC, CTTGTA, CCATGA
| &nbsp


|- style="background-color:#D9D9D9;font-size:12pt"
|- style="background-color:#D9D9D9;font-size:12pt"
Line 118: Line 122:
|style="text-decoration:none" align="center" | 16-20
|style="text-decoration:none" align="center" | 16-20
|  | GGGGTT, CTCATC, AGTTCA, GACGCA, CCTGTC
|  | GGGGTT, CTCATC, AGTTCA, GACGCA, CCTGTC
| &nbsp


|- style="font-size:12pt"
|- style="font-size:12pt"
Line 124: Line 129:
| align="center" align="center" valign="bottom" | 21,22,23,26,27
| align="center" align="center" valign="bottom" | 21,22,23,26,27
| valign="bottom" | CAACCC,CTCGGA,TCGAAA,TCGTGA,TACGCG
| valign="bottom" | CAACCC,CTCGGA,TCGAAA,TCGTGA,TACGCG
| &nbsp


|- style="font-size:12pt"
|- style="font-size:12pt"
Line 130: Line 136:
| align="center" valign="bottom" | 9-13
| align="center" valign="bottom" | 9-13
| valign="bottom" | GATCAG,TAGCTT,GGCTAC,CTTGTA,CCATGA
| valign="bottom" | GATCAG,TAGCTT,GGCTAC,CTTGTA,CCATGA
| &nbsp


|- style="font-size:12pt"
|- style="font-size:12pt"
Line 136: Line 143:
| align="center" valign="bottom" | 10-13
| align="center" valign="bottom" | 10-13
| valign="bottom" | TAGCTT,GGCTAC,CTTGTA,CCATGA
| valign="bottom" | TAGCTT,GGCTAC,CTTGTA,CCATGA
| &nbsp


|- style="font-size:12pt"
|- style="font-size:12pt"
Line 142: Line 150:
| align="center" valign="bottom" | 33,41,42,44
| align="center" valign="bottom" | 33,41,42,44
| valign="bottom" | CGCAAC,GGGAAC,GAAAAG,GCATCC
| valign="bottom" | CGCAAC,GGGAAC,GAAAAG,GCATCC
| &nbsp


|- style="font-size:12pt"
|- style="font-size:12pt"
Line 148: Line 157:
| align="center" valign="bottom" | 34-36
| align="center" valign="bottom" | 34-36
| valign="bottom" | AACTGA,ATCGGG,CAAGCA
| valign="bottom" | AACTGA,ATCGGG,CAAGCA
| &nbsp


|- style="font-size:12pt"
|- style="font-size:12pt"
Line 154: Line 164:
| align="center" valign="bottom" | 24,25
| align="center" valign="bottom" | 24,25
| valign="bottom" | ATTCGC,TACTGC
| valign="bottom" | ATTCGC,TACTGC
| &nbsp


|- style="font-size:12pt"
|- style="font-size:12pt"
Line 160: Line 171:
| align="center" valign="bottom" | 30,31
| align="center" valign="bottom" | 30,31
| valign="bottom" | TTAGTG,CACCAC
| valign="bottom" | TTAGTG,CACCAC
| align="center" | [[Daniel:Notebook/ComboLock/2016-11-18|11/18/16]], [[Daniel:Notebook/ComboLock/2016-11-21|11/21/16]]




Line 167: Line 179:
| align="center" valign="bottom" | 25
| align="center" valign="bottom" | 25
| valign="bottom" | TACTGC
| valign="bottom" | TACTGC
| align="center" | [[Daniel:Notebook/ComboLock/2016-11-21|11/21/16]]


|}
|}

Revision as of 20:55, 21 November 2016

Sequencing Lists

Back to Notebook

This page describes the sequencing samples submitted in the ComboLock project. It keeps track of IDs, etc.

Zhang Lab Indexes

Sequencing Table

Expected length of combolock amplicons with total sequencing length for the forward and reverse directions

Name Length (bp) Forward Seq. Length Reverse Seq. Length
LinkerL 18 18 148
Primer2 18 36 130
BC-C1 8 44 112
Primer4 18 62 104
BCLatch 8 70 86
UMI 8 78 78
Primer6 18 96 70
BC-C2 8 104 52
Primer12 18 122 44
BCPadlock 8 130 26
LinkerR 18 148 18

Master List

Sample Name Experiment Start Date Indexes Index Sequences Results
DEJ20160630_CLCProbe_Test2 6/23/16 2,3,5,6 CGATGT, TTAGGC, ACAGTG, GCCAAT &nbsp
DEJ20160706_CL_P01 6/28/16 1-7 ATCACG, CGATGT, TTAGGC, TGACCA, ACAGTG, GCCAAT, CAGATC &nbsp
DEJ20160706_CL_C02 6/29/16 10-13 TAGCTT, GGCTAC, CTTGTA, CCATGA &nbsp
DEJ20160715_C03 7/11/16 16-20 GGGGTT, CTCATC, AGTTCA, GACGCA, CCTGTC &nbsp
DEJ20160722_CL_C04 7/18/16 21,22,23,26,27 CAACCC,CTCGGA,TCGAAA,TCGTGA,TACGCG &nbsp
DEJ20160727_CL_C05 7/25/16 9-13 GATCAG,TAGCTT,GGCTAC,CTTGTA,CCATGA &nbsp
DEJ20160822_CL_PC01 8/18/16 10-13 TAGCTT,GGCTAC,CTTGTA,CCATGA &nbsp
DEJ20160826_CL_PC02 8/22/16 33,41,42,44 CGCAAC,GGGAAC,GAAAAG,GCATCC &nbsp
DEJ20160906_CL_PC03 8/29/16 34-36 AACTGA,ATCGGG,CAAGCA &nbsp
DEJ201610_CL_PCC01 10/07/16 24,25 ATTCGC,TACTGC &nbsp
DEJ-CL-RCA01-20161103 10/31/16 30,31 TTAGTG,CACCAC 11/18/16, 11/21/16


DEJ-CL-CellC01-20161116 11/7/16 25 TACTGC 11/21/16

Sequencing Runs 2016

Summer 2016

07-08-2016

Sample Name Experiment Start Date Indexes Index Sequences
DEJ20160630_CLCProbe_Test2 6/23/16 2,3,5,6 CGATGT, TTAGGC, ACAGTG, GCCAAT
DEJ20160706_CL_P01 6/28/16 1-7 ATCACG, CGATGT, TTAGGC, TGACCA, ACAGTG, GCCAAT, CAGATC
DEJ20160706_CL_C02 6/29/16 10-13 TAGCTT, GGCTAC, CTTGTA, CCATGA

07-15-2016

Sample Name Experiment Start Date Indexes Index Sequences
DEJ20160630_CLCProbe_Test2 6/23/16 2,3,5,6 CGATGT, TTAGGC, ACAGTG, GCCAAT
DEJ20160706_CL_P01 6/28/16 1-7 ATCACG, CGATGT, TTAGGC, TGACCA, ACAGTG, GCCAAT, CAGATC
DEJ20160706_CL_C02 6/29/16 10-13 TAGCTT, GGCTAC, CTTGTA, CCATGA
DEJ20160715_C03 7/11/16 16-20 GGGGTT, CTCATC, AGTTCA, GACGCA, CCTGTC

08-22-2016

Sample Name Experiment Start Date Indexes Index Sequences Results
DEJ20160822_CL_PC01 8/18/16 10-13 TAGCTT,GGCTAC,CTTGTA,CCATGA Analysis and Results

08-26-2016

Sample Name Experiment Start Date Indexes Index Sequences Results
DEJ20160826_CL_PC02 8/22/16 33, 40-42 CGCAAC,TGACGG,GGGAAC,GAAAAG 9/12/16

Fall 2016

09-12-2016

Sample Name Experiment Start Date Indexes Index Sequences Results
DEJ20160906_CL_PC03 8/29/16 34-36 AACTGA,ATCGGG,CAAGCA 9/27/16

10-18-2016

Sample Name Experiment Start Date Indexes Index Sequences Results
DEJ20161013_CL_PCC01 10/7/16 24,25 ATTCGC,TACTGC 10/20/16

11-10-2016

Sample Name Experiment Start Date Indexes Index Sequences Results
DEJ-CL-RCA01-20161103 10/31/16 30,31 TTAGTG,CACCAC 11/18/16

11-17-2016

Sample Name Experiment Start Date Indexes Index Sequences Results
DEJ-CL-CellC01-20161116 11/7/16 25 TACTGC 11/21/16
DEJ-CL-RCA01-20161103 10/31/16 30,31 TTAGTG,CACCAC 11/21/16