Daniel:Notebook/ComboLock/Sequencing: Difference between revisions
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| width="127" | Indexes | | width="127" | Indexes | ||
| width="300" | Index Sequences | | width="300" | Index Sequences | ||
| width="150" | Results | |||
|- style="font-size:12pt" | |- style="font-size:12pt" | ||
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|style="text-decoration:none" align="center" align="center" | 2,3,5,6 | |style="text-decoration:none" align="center" align="center" | 2,3,5,6 | ||
| | CGATGT, TTAGGC, ACAGTG, GCCAAT | | | CGATGT, TTAGGC, ACAGTG, GCCAAT | ||
|   | |||
|- style="background-color:#D9D9D9;font-size:12pt" | |- style="background-color:#D9D9D9;font-size:12pt" | ||
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|style="text-decoration:none" align="center" | 1-7 | |style="text-decoration:none" align="center" | 1-7 | ||
| | ATCACG, CGATGT, TTAGGC, TGACCA, ACAGTG, GCCAAT, CAGATC | | | ATCACG, CGATGT, TTAGGC, TGACCA, ACAGTG, GCCAAT, CAGATC | ||
|   | |||
|- style="font-size:12pt" | |- style="font-size:12pt" | ||
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|style="text-decoration:none" align="center" | 10-13 | |style="text-decoration:none" align="center" | 10-13 | ||
| | TAGCTT, GGCTAC, CTTGTA, CCATGA | | | TAGCTT, GGCTAC, CTTGTA, CCATGA | ||
|   | |||
|- style="background-color:#D9D9D9;font-size:12pt" | |- style="background-color:#D9D9D9;font-size:12pt" | ||
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|style="text-decoration:none" align="center" | 16-20 | |style="text-decoration:none" align="center" | 16-20 | ||
| | GGGGTT, CTCATC, AGTTCA, GACGCA, CCTGTC | | | GGGGTT, CTCATC, AGTTCA, GACGCA, CCTGTC | ||
|   | |||
|- style="font-size:12pt" | |- style="font-size:12pt" | ||
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| align="center" align="center" valign="bottom" | 21,22,23,26,27 | | align="center" align="center" valign="bottom" | 21,22,23,26,27 | ||
| valign="bottom" | CAACCC,CTCGGA,TCGAAA,TCGTGA,TACGCG | | valign="bottom" | CAACCC,CTCGGA,TCGAAA,TCGTGA,TACGCG | ||
|   | |||
|- style="font-size:12pt" | |- style="font-size:12pt" | ||
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| align="center" valign="bottom" | 9-13 | | align="center" valign="bottom" | 9-13 | ||
| valign="bottom" | GATCAG,TAGCTT,GGCTAC,CTTGTA,CCATGA | | valign="bottom" | GATCAG,TAGCTT,GGCTAC,CTTGTA,CCATGA | ||
|   | |||
|- style="font-size:12pt" | |- style="font-size:12pt" | ||
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| align="center" valign="bottom" | 10-13 | | align="center" valign="bottom" | 10-13 | ||
| valign="bottom" | TAGCTT,GGCTAC,CTTGTA,CCATGA | | valign="bottom" | TAGCTT,GGCTAC,CTTGTA,CCATGA | ||
|   | |||
|- style="font-size:12pt" | |- style="font-size:12pt" | ||
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| align="center" valign="bottom" | 33,41,42,44 | | align="center" valign="bottom" | 33,41,42,44 | ||
| valign="bottom" | CGCAAC,GGGAAC,GAAAAG,GCATCC | | valign="bottom" | CGCAAC,GGGAAC,GAAAAG,GCATCC | ||
|   | |||
|- style="font-size:12pt" | |- style="font-size:12pt" | ||
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| align="center" valign="bottom" | 34-36 | | align="center" valign="bottom" | 34-36 | ||
| valign="bottom" | AACTGA,ATCGGG,CAAGCA | | valign="bottom" | AACTGA,ATCGGG,CAAGCA | ||
|   | |||
|- style="font-size:12pt" | |- style="font-size:12pt" | ||
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| align="center" valign="bottom" | 24,25 | | align="center" valign="bottom" | 24,25 | ||
| valign="bottom" | ATTCGC,TACTGC | | valign="bottom" | ATTCGC,TACTGC | ||
|   | |||
|- style="font-size:12pt" | |- style="font-size:12pt" | ||
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| align="center" valign="bottom" | 30,31 | | align="center" valign="bottom" | 30,31 | ||
| valign="bottom" | TTAGTG,CACCAC | | valign="bottom" | TTAGTG,CACCAC | ||
| align="center" | [[Daniel:Notebook/ComboLock/2016-11-18|11/18/16]], [[Daniel:Notebook/ComboLock/2016-11-21|11/21/16]] | |||
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| align="center" valign="bottom" | 25 | | align="center" valign="bottom" | 25 | ||
| valign="bottom" | TACTGC | | valign="bottom" | TACTGC | ||
| align="center" | [[Daniel:Notebook/ComboLock/2016-11-21|11/21/16]] | |||
|} | |} |
Revision as of 20:55, 21 November 2016
Sequencing Lists
This page describes the sequencing samples submitted in the ComboLock project. It keeps track of IDs, etc.
Sequencing Table
Expected length of combolock amplicons with total sequencing length for the forward and reverse directions
Name | Length (bp) | Forward Seq. Length | Reverse Seq. Length |
LinkerL | 18 | 18 | 148 |
Primer2 | 18 | 36 | 130 |
BC-C1 | 8 | 44 | 112 |
Primer4 | 18 | 62 | 104 |
BCLatch | 8 | 70 | 86 |
UMI | 8 | 78 | 78 |
Primer6 | 18 | 96 | 70 |
BC-C2 | 8 | 104 | 52 |
Primer12 | 18 | 122 | 44 |
BCPadlock | 8 | 130 | 26 |
LinkerR | 18 | 148 | 18 |
Master List
Sample Name | Experiment Start Date | Indexes | Index Sequences | Results |
DEJ20160630_CLCProbe_Test2 | 6/23/16 | 2,3,5,6 | CGATGT, TTAGGC, ACAGTG, GCCAAT |   |
DEJ20160706_CL_P01 | 6/28/16 | 1-7 | ATCACG, CGATGT, TTAGGC, TGACCA, ACAGTG, GCCAAT, CAGATC |   |
DEJ20160706_CL_C02 | 6/29/16 | 10-13 | TAGCTT, GGCTAC, CTTGTA, CCATGA |   |
DEJ20160715_C03 | 7/11/16 | 16-20 | GGGGTT, CTCATC, AGTTCA, GACGCA, CCTGTC |   |
DEJ20160722_CL_C04 | 7/18/16 | 21,22,23,26,27 | CAACCC,CTCGGA,TCGAAA,TCGTGA,TACGCG |   |
DEJ20160727_CL_C05 | 7/25/16 | 9-13 | GATCAG,TAGCTT,GGCTAC,CTTGTA,CCATGA |   |
DEJ20160822_CL_PC01 | 8/18/16 | 10-13 | TAGCTT,GGCTAC,CTTGTA,CCATGA |   |
DEJ20160826_CL_PC02 | 8/22/16 | 33,41,42,44 | CGCAAC,GGGAAC,GAAAAG,GCATCC |   |
DEJ20160906_CL_PC03 | 8/29/16 | 34-36 | AACTGA,ATCGGG,CAAGCA |   |
DEJ201610_CL_PCC01 | 10/07/16 | 24,25 | ATTCGC,TACTGC |   |
DEJ-CL-RCA01-20161103 | 10/31/16 | 30,31 | TTAGTG,CACCAC | 11/18/16, 11/21/16
|
DEJ-CL-CellC01-20161116 | 11/7/16 | 25 | TACTGC | 11/21/16 |
Sequencing Runs 2016
Summer 2016
07-08-2016
Sample Name | Experiment Start Date | Indexes | Index Sequences |
DEJ20160630_CLCProbe_Test2 | 6/23/16 | 2,3,5,6 | CGATGT, TTAGGC, ACAGTG, GCCAAT |
DEJ20160706_CL_P01 | 6/28/16 | 1-7 | ATCACG, CGATGT, TTAGGC, TGACCA, ACAGTG, GCCAAT, CAGATC |
DEJ20160706_CL_C02 | 6/29/16 | 10-13 | TAGCTT, GGCTAC, CTTGTA, CCATGA |
07-15-2016
Sample Name | Experiment Start Date | Indexes | Index Sequences |
DEJ20160630_CLCProbe_Test2 | 6/23/16 | 2,3,5,6 | CGATGT, TTAGGC, ACAGTG, GCCAAT |
DEJ20160706_CL_P01 | 6/28/16 | 1-7 | ATCACG, CGATGT, TTAGGC, TGACCA, ACAGTG, GCCAAT, CAGATC |
DEJ20160706_CL_C02 | 6/29/16 | 10-13 | TAGCTT, GGCTAC, CTTGTA, CCATGA |
DEJ20160715_C03 | 7/11/16 | 16-20 | GGGGTT, CTCATC, AGTTCA, GACGCA, CCTGTC |
08-22-2016
Sample Name | Experiment Start Date | Indexes | Index Sequences | Results |
DEJ20160822_CL_PC01 | 8/18/16 | 10-13 | TAGCTT,GGCTAC,CTTGTA,CCATGA | Analysis and Results |
08-26-2016
Sample Name | Experiment Start Date | Indexes | Index Sequences | Results |
DEJ20160826_CL_PC02 | 8/22/16 | 33, 40-42 | CGCAAC,TGACGG,GGGAAC,GAAAAG | 9/12/16 |
Fall 2016
09-12-2016
Sample Name | Experiment Start Date | Indexes | Index Sequences | Results |
DEJ20160906_CL_PC03 | 8/29/16 | 34-36 | AACTGA,ATCGGG,CAAGCA | 9/27/16 |
10-18-2016
Sample Name | Experiment Start Date | Indexes | Index Sequences | Results |
DEJ20161013_CL_PCC01 | 10/7/16 | 24,25 | ATTCGC,TACTGC | 10/20/16 |
11-10-2016
Sample Name | Experiment Start Date | Indexes | Index Sequences | Results |
DEJ-CL-RCA01-20161103 | 10/31/16 | 30,31 | TTAGTG,CACCAC | 11/18/16 |
11-17-2016
Sample Name | Experiment Start Date | Indexes | Index Sequences | Results |
DEJ-CL-CellC01-20161116 | 11/7/16 | 25 | TACTGC | 11/21/16 |
DEJ-CL-RCA01-20161103 | 10/31/16 | 30,31 | TTAGTG,CACCAC | 11/21/16
|