Daniel:Notebook/ComboLock/2016-8-26: Difference between revisions

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(Created page with "=Positive Control Amplicon Test 2 (Started Monday)= Back to Calendar ==Protocol-Library Prep== <ol sta...")
 
>Djacobse
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|- style="font-size:12pt"
|- style="font-size:12pt"
| height="15"  valign="bottom" | 20160822 Sample A
| height="15"  valign="bottom" | 20160822 Sample A
| align="center" align="center" valign="bottom" | &nbsp;
| align="center" align="center" valign="bottom" | 38.4


|- style="background-color:#D9D9D9;font-size:12pt"
|- style="background-color:#D9D9D9;font-size:12pt"
| height="15"  valign="bottom" | 20160822 Sample B
| height="15"  valign="bottom" | 20160822 Sample B
| align="center" align="center" valign="bottom" | &nbsp;
| align="center" align="center" valign="bottom" | 30.1


|}
|}

Revision as of 17:29, 26 August 2016

Positive Control Amplicon Test 2 (Started Monday)

Back to Calendar

Protocol-Library Prep

  1. Ethanol Precipitation
    1. Centrifuge at 12000rpm at 4C for 25 minutes
    2. Remove supernatant and add 750 uL 70% EtOH
    3. Centrifuge at 12000rpm at 4C for 15 minutes
    4. Remove supernatant and let dry in PCR hood for 15 min
    5. Resuspend in 30 uL nfH2O

Nanodrop Results

Sample ng/uL ssDNA
20160822 Sample A 38.4
20160822 Sample B 30.1