Daniel:Notebook/ComboLock/2016-8-26: Difference between revisions
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>Djacobse (Created page with "=Positive Control Amplicon Test 2 (Started Monday)= Back to Calendar ==Protocol-Library Prep== <ol sta...") |
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|- style="font-size:12pt" | |- style="font-size:12pt" | ||
| height="15" valign="bottom" | 20160822 Sample A | | height="15" valign="bottom" | 20160822 Sample A | ||
| align="center" align="center" valign="bottom" | | | align="center" align="center" valign="bottom" | 38.4 | ||
|- style="background-color:#D9D9D9;font-size:12pt" | |- style="background-color:#D9D9D9;font-size:12pt" | ||
| height="15" valign="bottom" | 20160822 Sample B | | height="15" valign="bottom" | 20160822 Sample B | ||
| align="center" align="center" valign="bottom" | | | align="center" align="center" valign="bottom" | 30.1 | ||
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Revision as of 17:29, 26 August 2016
Positive Control Amplicon Test 2 (Started Monday)
Protocol-Library Prep
- Ethanol Precipitation
- Centrifuge at 12000rpm at 4C for 25 minutes
- Remove supernatant and add 750 uL 70% EtOH
- Centrifuge at 12000rpm at 4C for 15 minutes
- Remove supernatant and let dry in PCR hood for 15 min
- Resuspend in 30 uL nfH2O
Nanodrop Results
Sample | ng/uL ssDNA |
20160822 Sample A | 38.4 |
20160822 Sample B | 30.1 |