Daniel:Notebook/ComboLock/2016-8-26: Difference between revisions
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<li>Ethanol Precipitation</li> | <li>Ethanol Precipitation (from [[Daniel:Notebook/ComboLock/2016-8-25|yesterday]])</li> | ||
<ol type="A" start="3"> | <ol type="A" start="3"> | ||
<li>Centrifuge at 12000rpm at 4C for 25 minutes</li> | <li>Centrifuge at 12000rpm at 4C for 25 minutes</li> |
Revision as of 17:30, 26 August 2016
Positive Control Amplicon Test 2 (Started Monday)
Protocol-Library Prep
- Ethanol Precipitation (from yesterday)
- Centrifuge at 12000rpm at 4C for 25 minutes
- Remove supernatant and add 750 uL 70% EtOH
- Centrifuge at 12000rpm at 4C for 15 minutes
- Remove supernatant and let dry in PCR hood for 15 min
- Resuspend in 30 uL nfH2O
Nanodrop Results
Sample | ng/uL ssDNA |
20160822 Sample A | 38.4 |
20160822 Sample B | 30.1 |