Daniel:Notebook/ComboLock/2016-8-26: Difference between revisions

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>Djacobse
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<ol start="7">
<ol start="7">
<li>Ethanol Precipitation</li>
<li>Ethanol Precipitation (from [[Daniel:Notebook/ComboLock/2016-8-25|yesterday]])</li>
<ol type="A" start="3">
<ol type="A" start="3">
<li>Centrifuge at 12000rpm at 4C for 25 minutes</li>
<li>Centrifuge at 12000rpm at 4C for 25 minutes</li>

Revision as of 17:30, 26 August 2016

Positive Control Amplicon Test 2 (Started Monday)

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Protocol-Library Prep

  1. Ethanol Precipitation (from yesterday)
    1. Centrifuge at 12000rpm at 4C for 25 minutes
    2. Remove supernatant and add 750 uL 70% EtOH
    3. Centrifuge at 12000rpm at 4C for 15 minutes
    4. Remove supernatant and let dry in PCR hood for 15 min
    5. Resuspend in 30 uL nfH2O

Nanodrop Results

Sample ng/uL ssDNA
20160822 Sample A 38.4
20160822 Sample B 30.1