Daniel:Notebook/ComboLock/2016-9-6: Difference between revisions
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>Djacobse (Created page with "=X3 Dilution Test (8/29/2016)= Back to Calendar ==Protocol== <ol start="6"> <li>Ethanol Precipitation<...") |
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==Sequencing Library== | ==Sequencing Library== |
Revision as of 16:16, 9 September 2016
X3 Dilution Test (8/29/2016)
Protocol
- Ethanol Precipitation
- Pellet by centrifuging for 25 minutes at 12000rpm at 4C
- Remove supernatant and add 750 uL chilled 70% EtOH
- Centrifuge for 12 minutes at 12000rpm at 4C
- Remove supernatant and spin in vacuum centrifuge for 10 minutes
- Add 20 uL nfH2O and measure in nanodrop
- Combined 15 uL from each of the two tubes above
- Stored at 4C in upcoming libraries box
Nanodrop Results
Sample | ng/uL |
Sample A | 31 |
Sample B | 19.3 |
Sequencing Library
Sample | ng/uL | uL added | final mass (ng) |
9-6 Sample A | 31 | 15 | 465 |
9-6 Sample B | 19.3 | 15 | 289.5 |