Kun:LabNotes/CpgSeq/2007-12-4: Difference between revisions
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(New page: ==Exp.1 PAGE purification of padlock oligos== I received the 36 CpGMIPv2 oligos. I pooled 20ul of the first 24 oligos into the tube CpGMIPv2L, and 20ul of the remaining 12 oligos into the ...) |
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I made 1:10 dilution of the two mixtures (10ul into 90ul 1X TE), measure the concentration by Nanodrop. | I made 1:10 dilution of the two mixtures (10ul into 90ul 1X TE), measure the concentration by Nanodrop. | ||
CpGMIPv2L(10uM): 559ng/ul | CpGMIPv2L(10uM): 559ng/ul 3ul oligos + 9ul water + 12ul 2x Loading dye => 2ul per lane | ||
CpGMIPv2S(10uM): 459ng/ul | CpGMIPv2S(10uM): 459ng/ul 1.5ul oligos + 4.5ul water + 6ul 2x loading dye => 2ul per lane. |
Revision as of 01:33, 5 December 2007
Exp.1 PAGE purification of padlock oligos
I received the 36 CpGMIPv2 oligos. I pooled 20ul of the first 24 oligos into the tube CpGMIPv2L, and 20ul of the remaining 12 oligos into the tube CpGMIPv2S.
I made 1:10 dilution of the two mixtures (10ul into 90ul 1X TE), measure the concentration by Nanodrop.
CpGMIPv2L(10uM): 559ng/ul 3ul oligos + 9ul water + 12ul 2x Loading dye => 2ul per lane CpGMIPv2S(10uM): 459ng/ul 1.5ul oligos + 4.5ul water + 6ul 2x loading dye => 2ul per lane.