Kun:LabNotes/CpgSeq/2007-12-4: Difference between revisions

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(New page: ==Exp.1 PAGE purification of padlock oligos== I received the 36 CpGMIPv2 oligos. I pooled 20ul of the first 24 oligos into the tube CpGMIPv2L, and 20ul of the remaining 12 oligos into the ...)
 
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I made 1:10 dilution of the two mixtures (10ul into 90ul 1X TE), measure the concentration by Nanodrop.
I made 1:10 dilution of the two mixtures (10ul into 90ul 1X TE), measure the concentration by Nanodrop.
     CpGMIPv2L(10uM): 559ng/ul           2ul oligos + 10ul water + 12ul 2x Loading dye => 2ul per lane
     CpGMIPv2L(10uM): 559ng/ul   3ul oligos + 9ul water + 12ul 2x Loading dye => 2ul per lane
     CpGMIPv2S(10uM): 459ng/ul           1ul oligos + 5ul water + 6ul 2x loading dye => 2ul per lane.
     CpGMIPv2S(10uM): 459ng/ul   1.5ul oligos + 4.5ul water + 6ul 2x loading dye => 2ul per lane.

Revision as of 01:33, 5 December 2007

Exp.1 PAGE purification of padlock oligos

I received the 36 CpGMIPv2 oligos. I pooled 20ul of the first 24 oligos into the tube CpGMIPv2L, and 20ul of the remaining 12 oligos into the tube CpGMIPv2S.

I made 1:10 dilution of the two mixtures (10ul into 90ul 1X TE), measure the concentration by Nanodrop.

    CpGMIPv2L(10uM): 559ng/ul   3ul oligos + 9ul water + 12ul 2x Loading dye => 2ul per lane
    CpGMIPv2S(10uM): 459ng/ul   1.5ul oligos + 4.5ul water + 6ul 2x loading dye => 2ul per lane.