Daniel:Notebook/ComboLock/2016-9-29: Difference between revisions
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>Djacobse (Created page with "=Positive Control Amplicon Production (PCAmp4; Started yesterday)= Back to Calendar ==Protocol== <ol s...") |
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Revision as of 17:20, 29 September 2016
Positive Control Amplicon Production (PCAmp4; Started yesterday)
Protocol
- Size Selection
- Centrifuge at 12000 rpm for 1.5 minutes
- Extract the supernatant and place into NanoSep column
- Centrifuge at 12000 rpm for 1.5 minutes
- Ethanol Precipitation
- Move sample to a fresh 2 mL tube
- Add 1250 uL 100% EtOH, 50 uL 3M NaOAc and 2 uL glycoblue
- Store at for 6 hours
- Pellet by centrifuging for 25 minutes at 12000rpm at 4C
- Remove supernatant and add 750 uL chilled 70% EtOH
- Centrifuge for 12 minutes at 12000rpm at 4C
- Remove supernatant and spin in vacuum centrifuge for 10 minutes
- Add 50 uL nfH2O and resuspend every tube in series;
- Measure final concentration in nanodrop
Results
Sample | ng/uL ssDNA |
C4-C2 Amplicon (Final) |