Daniel:Notebook/ComboLock/2016-11-3: Difference between revisions
Jump to navigation
Jump to search
>Djacobse |
>Djacobse No edit summary |
||
Line 1: | Line 1: | ||
=Circularization Optimization Testing (Started [[Daniel:Notebook/ComboLock/2016-10-31|Monday]])= | |||
[[Daniel:Notebook/ComboLock|Back to Calendar]] | [[Daniel:Notebook/ComboLock|Back to Calendar]] | ||
==Library Prep (Started [[Daniel:Notebook/ComboLock/2016-11-2|Yesterday]])== | ==Library Prep (Started [[Daniel:Notebook/ComboLock/2016-11-2|Yesterday]])== |
Revision as of 18:25, 2 November 2016
Circularization Optimization Testing (Started Monday)
Library Prep (Started Yesterday)
- Ethanol Precipitation
- Centrifuge for 25 minutes at 12000 rpm at 4C
- Remove supernatant and add 750 uL 70% EtOH
- Centrifuge for 12 minutes at 12000 rpm at 4C
- Remove supernatant and spin in vacuum centrifuge for 10 minutes
- Add 30 (20 for NTC) uL nfH2O to resuspend; pool samples A-C by reusing the water